染色的干细胞CDy1 货号：E-14001
Stem Cell CDy1 Dye
背景资料：Active Motif's Stem Cell CDy1 Dye provides a fast and simple method to selectively stain live pluripotent stem cells for identification and sorting using fluorescence microscopy and FACS. Because CDy1 is a live cell dye, it eliminates the need to sacrifice precious stem cell samples for testing, providing a significant advantage over other methods such as immunostaining that require cell fixation. In comparison to other live cell staining techniques, CDy1 is highly specific for pluripotent stem cells and does not produce background signal in other cells, such as feeder or differentiated cells, like live alkaline phosphatase staining methods. Staining is reversible and does not impact cell growth, morphology, or differentiation, making it ideal for stem cell enrichment and sorting, or for performing additional experiments or counterstaining procedures.
产品描述：Scientists at the Agency for Science, Technology and Research (A*STAR) and the National University of Singapore first discovered the dye as a selective stain for pluripotent mouse and human embryonic stem cells (mESCs, hESCs) enabling the identification stem cells from feeder cells or mixed cultures.
产品使用： In these studies, the CDy1 dye was also utilized to determine reprogramming of induced pluripotent stem cells (iPSC) following retroviral infection of the Oct4, Sox2, Klf4 and c-Myc transcription factors. Their studies were published in Nature Protocols in 20111. Subsequent studies validating the use of the Stem Cell CDy1 dye for identification and selection of other stem cell types have also been published.
产品应用：.
In a recent publication from the laboratory of Geoffrey W. Osborne at the University of Queensland, the Stem Cell CDy1 dye was used to enrich for neural stem cells from mouse brain2. Also, studies from the laboratory of Robert Hawley demonstrate the use of the Stem Cell CDy1 dye to identify a subpopulation of drug-resistant Multiple Myeloma (MM) cells believed to be cancer stem cells2. To see detailed information on published data utilizing the CDy1 Dye, please click on the References tab below.
文献参考：
- Kang, N.Y. et al. (2011) Nat. Protoc. 6, 1044-1052.
- Vukovic, J. et al. (2013) Stem Cells Dev. doi:10.1089/scd.2012.0660.
- Hawley, T.S. et al. (2013) Am J Hematol. 88, 265-272.
定购信息：
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
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