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RNA |
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RNA>MicroRNA/mRNA/cfRNA |
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micro RNA极速提取试剂盒(A-R5301) |
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micro RNA极速提取试剂盒
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快速——30分钟完成实验。 |
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高效分离小RNA,同时分离总RNA |
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富集200nt以下的小RNA,增加其用于下游实验的浓度。 |
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可用于miRNA、siRNA、shRNA和snRNA分析。 |
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Format: Spin Column |
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Sample Sources: RNA fragments (≥17 nucleotides), labeled RNA fragments, in vitro transcription products, etc.还适用于各种来源的样品。 |
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microRNA Recovery: Typically, RNA is eluted into as little as 6-10 µl RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the supplied RB Columns is ~5 µg. |
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microRNA Purity: High quality microRNA (A260/A280 > 1.8, A260/A230 > 1.8) suitable for all downstream RNA-based manipulations. |
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microRNA Storage: microRNA is eluted with RNase-free water and can be stored at ≤ -70°C. The addition of RNase inhibitors is optional but highly recommended for prolonged storage. |
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Equipment Needed: Microcentrifuge for ex.eppendorf 5415c |
micro RNA极速提取试剂盒 provides a simple and reliable method for the rapid preparation of up to ~5 μg (per prep.) of high-quality RT-PCR-ready, DNA-free RNA. This simple procedure is based on the use of a unique single-buffer system and Fast-Spin column technology. The procedure is easy: treat your RNA sample with DNase I, add binding buffer, adjust the conditions by adding ethanol, and the cleaned RNA is then concentrated into ≥6 μl of RNase-free water using the RB Column. RNA fragments (≥17 bases) can be safely treated and recovered using this kit. The result is highlyconcentrated, purified RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, etc. The purification procedure after the DNase treatment typically takes about 5 minutes.
Purification of RNA samples containing genomic and plasmid DNA by using the micro RNA极速提取试剂盒. (1) RNA/DNA sample purified without prior DNase treatment, (2) DNase treated, purified RNA sample.
Product Name |
Catalog No. |
Size |
Price(RMB) |
Protocol |
micro RNA极速提取试剂盒 |
A-R5301 |
50 次 |
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A-R5302 |
200 次 |
RT-PCR相关产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
Thermo M-MLV 反转录酶 |
10000U |
PDF |
A-P6801 |
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Thermo M-MLV 反转录酶 |
50000U |
PDF |
A-P6802 |
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Supermo III M-MLV 反转录酶 |
10000U |
PDF |
A-P6811 |
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Supermo III M-MLV 反转录酶 |
50000U |
PDF |
A-P6812 |
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Super M-MLV 反转录酶 |
10000U |
PDF |
A-P6502 |
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Super M-MLV 反转录酶 |
50000U |
PDF |
A-P6503 |
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