Epiquick qPCR快速试剂盒 货号:A-P-1029
EpiQuik Quantitative PCR Fast Kit
背景资料:
The EpiQuik™ Quantitative PCR Fast Kit allows for a fast, specific, sensitive, and reproducible quantitative real time analysis of DNA samples. The novel hot start DNA polymerase contained in this kit reduces the overall time required for quantitative PCR from approximately 2 hours to just 70 minutes. The kit also facilitates sensitivity and specificity of quantitative PCR by significantly increasing primer-DNA template annealing, while simultaneously reducing non-specific annealing. This results in considerable time savings and a more efficient real time PCR.
产品描述:
An extremely fast procedure that can be finished within 70 minutes.
Abundant yields due to high amplification efficiency.
Highly accurate and specific in PCR, which reduces false-positive results.
Convenient master mix format allows for an easy reaction setup.
Can be used with any block-based real-time PCR instrument.
Simple, reliable, and consistent assay conditions.
Principle & Procedure
The EpiQuik™ Quantitative PCR Fast Kit provides a master mix format which contains a hot start DNA polymerase, dNTPs, an MS-PCR enhancer, an optimized buffer, and an intercalating green dye. This master mix allows for a convenient and easy reaction setup. The unique hot start DNA polymerase is provided in an inactive state at ambient temperature and is reactivated by several minute long incubations at 95°C, which can easily be integrated into existing thermal cycling steps. The hot start DNA polymerase in combination with the optimized buffer ensures the quantitative PCR specificity and sensitivity. The green dye allows for DNA detection and analysis without using a sequence-specific probe.
产品特点:
1·极其快速的试剂盒,70分钟内完成反应;
2·高扩增效率获得丰富的产物;
3·高精确度和对MSP的特异性,降低假阳性概率;
4·方便的混合缓冲液模式使得配置反应体系更加简单;
5·操作简便、结果可靠、统一的分析条件;
6·可用于所有基于模块的实时PCR设备;
图片展示:
实验流程
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Epiquick qPCR快速试剂盒步骤图示
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参考文献:
Todorova K et. al. (January 2017). Micro-RNA-204 Participates in TMPRSS2/ERG Regulation and Androgen Receptor Reprogramming in Prostate Cancer. Horm Cancer.
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
Epiquick qPCR快速试剂盒 |
100 次 |
英文PDF-中文PDF |
A-P-1029-100 |
|
Epiquick qPCR快速试剂盒 |
200 次 |
英文PDF-中文PDF |
A-P-1029-200 |
|
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