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Epigenetics |
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| Epigenetics>【SBI】 |
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现货---EZ DNA Methylation-Lightning™ Kit(A-D5030 A-D5031) |
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Fastest method for complete bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
| EZ DNA Methlyation-Lightning Kit |
50 rxns. |
A-D5030 |
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| EZ DNA Methlyation-Lightning Kit |
200 rxns. |
A-D5031 |
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About EZ DNA Methylation-Lightning™ Kit
The EZ DNA Methylation-Lightning™ Kit features rapid and reliable bisulfite treatment and conversion of DNA for methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed using a unique low-elution spin column. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
Specifications
| DNA Recovery |
>80% |
| Conversion Efficiency |
>99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines. |
| Optimal DNA Input |
Samples containing between 100 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
DNA sequencing results following bisulfite treatment. DNA with methylated C at nucleotide position #5 was processed using the EZ DNA Methylation™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remains intact while the unmethylated cytosines at positions #7, 9, 11, 14 and 15 are completely converted into uracil following bisulfite treatment (detected as thymine following PCR).
Methylation Plot From Reduced Representation Bisulfite Sequencing (RRBS). Data shows the relative percentage of methylation at individual CpG sites in mouse DNA. Methylation percentage is shown across a ~3 Mb region of mouse chromosome 19. Bisulfite sequencing libraries were prepared using mouse genomic DNA prepped with the Genomic Clean & Concentrator™ (D4010, D4011 – Zymo Research) and bisulfite converted using EZ DNA Methylation™ technology prior to Next-Gen sequencing.
Zymo-Spin™ IC Design Characteristics. The image above shows the unique design of the Zymo column that facilitates extremely small elution volumes (≥10 μl) without buffer carryover. This is unlike other columns that can retain liquid (binding/wash buffer residue) leading to carryover into the eluate.
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