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Epigenetics |
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| Epigenetics>【SBI】 |
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EZ-96 DNA Methylation-Lightning™ MagPrep(A-D5046 A-D5047) |
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EZ-96 DNA Methylation-Lightning™ MagPrep 货号：A-D5046
High-throughput procedure for automated rapid and complete bisulfite conversion of DNA for methylation analysis.
Ready-to-use conversion reagent is added directly to DNA.
High-yield, converted DNA is ideal for PCR, MSP, array, bisulfite and Next-Gen sequencing.
| EZ-96 DNA Methylation-Lightning MagPrep |
4x96 rxns. |
A-D5046 |
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| EZ-96 DNA Methylation-Lightning MagPrep |
8x96 rxns. |
A-D5047 |
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About EZ-96 DNA Methylation-Lightning™ MagPrep
The EZ-96 DNA Methylation-Lightning™ MagPrep Kit features rapid and reliable bisulfite treatment and conversion of DNA coupled to a magnetic bead based clean-up for high-throughput methylation analysis. Key to the fast workflow is the ready-to-use Lightning Conversion Reagent. No preparation is necessary, simply add this unique reagent to a DNA sample, wait about an hour, and let the reaction proceed to completion. DNA denaturation and bisulfite conversion processes are combined with added heat to facilitate rapid denaturation. Desulphonation and clean-up of the converted DNA is performed while bound to the MagBinding Beads. High yield, converted DNA is ideal for PCR, array, bisulfite and next generation sequencing, etc.
Specifications
| Conversion Efficiency |
>99.5% of non-methylated cytosine residues are converted to uracil; >99.5% protection of methylated cytosines. |
| Optimal DNA Input |
Samples containing between 100 pg to 2 μg of DNA. For optimal results, the amount of input DNA should be from 200 to 500 ng. |
DNA sequencing results following bisulfite treatment. DNA with methylated C at nucleotide position #5 was processed using the EZ DNA Methylation™ Kit. The recovered DNA was amplified by PCR and then sequenced directly. The methylated cytosine at position #5 remains intact while the unmethylated cytosines at positions #7, 9, 11, 14 and 15 are completely converted into uracil following bisulfite treatment (detected as thymine following PCR).
Methylation Plot From Reduced Representation Bisulfite Sequencing (RRBS). Data shows the relative percentage of methylation at individual CpG sites in mouse DNA. Methylation percentage is shown across a ~3 Mb region of mouse chromosome 19. Bisulfite sequencing libraries were prepared using mouse genomic DNA prepped with the Genomic Clean & Concentrator™ (D4010, D4011 – Zymo Research) and bisulfite converted using EZ DNA Methylation™ technology prior to Next-Gen sequencing.
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