EpiSonic 多功能生物处理系统                         货号：H5000-1

EpiSonic™ Multi-Functional Bioprocessor 1100 system

The EpiSonic™ Multi-Functional Bioprocessor 1100 system, an upgrade from the previous EpiSonic™ 1000 model, is a high-throughput capable sonication instrument of the latest generation for use in a wide range of biological applications, in particular for DNA and chromatin shearing. This completely digital instrument allows for processing of 1 to 96 samples or more, simultaneously, and can be easily integrated into existing lab workflows.

Technological Advantages
Several new aspects have been introduced since the earlier 1000 model to enhance the performance of the EpiSonic™.

The Working Principle
The technology that drives the EpiSonic™ 1100 so well is Digitally Adaptive Sonocavitation™ (DAS), a principle that is ideal for shearing DNA or chromatin as well as for lysing cells and tissues. During the DAS™ process, the electronic signal produced by a digitally-controlled generator is converted into acoustic/mechanical energy through a piezoelectric converter (also known as a transducer). When the acoustic energy is produced in water, negative pressure is generated and causes the distance between the water molecules to exceed the maximum molecular distance necessary to hold liquid intact. Consequently, the liquid breaks down to create millions of cavitation bubbles.

These cavitation bubbles expand in size through several acoustic cycles as more acoustic energy is introduced. The size of the cavitation bubbles and the corresponding cavitation energy can be manipulated and digitally controlled by adjusting fully scaled amplitude levels (1-100% in intervals of 1%) with a DAS™-based device such as the EpiSonic™ 1100. When the acoustic energy is suddenly removed, these cavitation bubbles collapse, creating intense shock waves in an extremely short period of time (microseconds), which transmit into vessels containing samples. This in turn forms highly targeted shearing forces to break up or disperse biomolecular samples including DNA, chromatin, and tissues in a non-contact, non-invasive manner. In a nutshell, a DAS™-based device such as the EpiSonic™ 1100 is able to shear samples with more precision and reproducibility than non-DAS™ instruments.

Compatible Sequencing Platforms
The EpiSonic™ 1100 can shear high quality DNA into any size under 2000 base pairs, which essentially covers all next-generation sequencing devices that require DNA library preparation. To be specific, some of the popular platforms that are compatible include the following:

Illumina Genome Analyzer

Illumina HiSeq

Illumina MiSeq

Roche 454 Sequencing GS FLX

Roche 454 Sequencing GS Junior

Life Technologies SOLiD System

The internal circuitry has been modified to apply DAS™ technology to allow for further precision and reproducibility

The redesigned, double-concave shaped sample processing horn causes piezoelectric vibration to propagate more evenly through the entire liquid medium (water) within the reservoir so that acoustic energy/mechanical stress is equally transmitted into samples located in different positions

A new vessel rack is conveniently included for pre-optimized operation with sample position consistency and run-to-run reproducibility

The included, new recirculating chiller promotes an isothermal environment to maintain the integrity of sensitive samples and prevent thermal degradation

Better optimized protocols included to shear DNA into various lengths for ideal compatibility with library preparation in next generation sequencing platforms from Illumina, Roche, and Life Technologies

Proven Precision, Consistency, & Reproducibility
The EpiSonic™ 1100 has been extensively tested and optimized for shearing samples in a precise, consistent, and reproducible manner. Desired sample sizes are able to be achieved due to the digital precision of the amplitude controls by allowing for user adjustment in intensity intervals of 1% at a time [Fig. 2]. Furthermore, samples placed in different positions with the included vessel holder (Tube Rack) are able to maintain consistency and equal sizes between each other [Fig. 3]. Most importantly, samples are sheared in reproducible lengths between the first run and the next run, creating predictable results and eliminating optimization time and labor [Fig. 4].

Features
The EpiSonic™ 1100 is a highly advanced ultrasonic instrument that incorporates Digitally Adaptive Sonocavitation™ (DAS) technology for efficiently processing biological samples with the following key features:

Touch Screen Control - Simple, modern operation with intuitive menu navigation

High Degree of Precision - Adjust amplitude levels 1% at a time, between 1% and 100%

Programmable Memory - Store successful protocols for future use

Chilled Environment - Included cooling system recirculates chilled water to maintain sample integrity and prevent thermal degradation

High Throughput - Simultaneously process from 1 to 96 samples with standard 0.2 ml PCR single tubes or 96-well PCR plates (Plate Rack sold separately)

Cost-Saving - Capable of handling common 0.2 ml PCR tubes or semi-skirted PCR plates, as well as low-priced EpiSonic-provided consumables

Shallow Waterbath - Less liquid medium allows for faster and highly focused sonication on samples

Closed Vessel Processing - Non-contact sonication in sealed tubes or wells prevents contamination and sample loss

Scalability - Simple, removable, and interchangeable vessel rack allows for expansion to different vessel sizes

Fast Processing - Shear 96 DNA samples to 100-500 bp (average 300 bp) fragments in just 10-20 minutes

Real-Time Energy Monitoring - Live screen output of amplitude, wattage, and joule readings

Real-Time Temperature Monitoring - Live screen output of temperature in sample reservoir (temperature probe required)

Improved Bio-Safety - No aerosol formation; no tedious manipulation of probes

Workflow Integration - Streamlines into high-throughput or multi-sample chromatin immunoprecipitation (ChIP) and massively parallel sequencing workflows

Automatic Overload Prevention - Detects faults and shuts down to prevent damage to circuits

No Moving Parts - Eliminates any possibility of mechanical failure or breakdown

Extensive Warranty - Backed by a 20 month warranty to ensure quality and customer satisfaction

Applications
The EpiSonic™ 1100 was designed and built with the processing of biological samples specifically in mind.

DNA Shearing

Chromatin Shearing

Cell Disruption/Lysis 

Life Technologies Ion Torrent

Product Specifications

Product Comparison

* Included tube rack is capable of optionally holding up to 81 additional sample tubes along with the 96 pre-optimized sample positions for a total of 177.

产品名称	描述	规格	价格	操作手册	货号
EpiSonic 多功能生物处理系统		1台		PDF	H5000-1