| 密理博（Millipore）公司自2006年收购知名的生物抗体试剂公司Serologicals (旗下有Upstate， Chemicon，Lincon，Celliance 等知名品牌)后，一直关注生命科学特别是表观遗传学和干细胞等前沿领域的研究。作为世界上第一个ChIP kit（货号17-95）的供应商，其表观遗传学研究相关产品一直处于世界领先地位。
DNA甲基化研究试剂
DNA甲基化（DNA methylation）是最早发现的表观遗传修饰途径之一，大量研究表明，DNA甲基化能引起染色质结构、DNA构象、DNA稳定性及DNA与蛋白质相互作用方式的改变，从而控制基因表达。
重亚硫酸盐甲基化DNA分析处理试剂盒
检测DNA甲基化的方法有很多种，在众多方法中，研究人员常常会使用到一项技术，那就是重亚硫酸氢盐转化。DNA经过重亚硫酸盐（Bisulfite）处理后，甲基化的胞嘧啶不发生变化，而未甲基化的胞嘧啶变为尿嘧啶。随后用测序、定量PCR和芯片等分析来比较处理和未处理DNA的序列，就能确定哪些碱基是甲基化的。默克密理博公司推出的极速重亚硫酸盐处理试剂盒（CpGenome™ Turbo Bisulfite Modification Kit），能在90分钟内完成DNA重亚硫酸盐处理及DNA纯化，修饰转化效率高达99.9%。
| 供应商 |
产品名称 |
规格 |
编号 |
试剂盒优点 |
| Millipore |
CpGenome Turbo Bisulfite Modification Kit |
50 rxns |
S7847 |
灵敏度: 1ng-1ug DNA含DNA纯化柱，转化率达99.9%，仅需90分钟，速度与效果、灵敏度并存 |
甲基化特异性PCR（methylation-specific PCR, MSP）试剂盒
甲基化特异性的PCR是一种灵敏度高且操作相对简单的甲基化研究方法。重亚硫酸盐处理DNA后，基因组DNA发生的由甲基化状态决定的序列改变，随后进行引物特异性的PCR。该方法引物设计是关键。MSP中设计两对引物，即一对结合处理后的甲基化DNA链（引物对M），另一对结合处理后的非甲基化DNA链（引物对U）。检测MSP扩增产物，如果用引物M能扩增出片段，则说明检测位点存在甲基化；若用引物U扩增出片段，则说明被检测的位点不存在甲基化。Millipore公司的CpG WIZ ®甲基化特异性PCR试剂盒，提供了目的基因的甲基化和非甲基化引物以及被验证的PCR条件，同时提供对照DNA。
| 供应商 |
产品名称 |
规格 |
编号 |
| Merck Millipore |
CPG WIZ OCT-4 AMPLIFICATION KIT |
25 assays |
S7840 |
| Merck Millipore |
CPG WIZE-CADHERIN AMPLIFICATION KIT |
25 assays |
S7804 |
| Merck Millipore |
CPG WIZ SOCS1 AMPLIFICATION KIT |
25 assays |
S7809 |
| Merck Millipore |
CPG WIZ ERALPHA AMPLIFICATION KIT |
25 assays |
S7815 |
| Merck Millipore |
CPG WIZ BRCA1 AMPLIFICATION KIT |
25 assays |
S7830 |
甲基化DNA分离纯化试剂盒
| 供应商 |
产品名称 |
规格 |
编号 |
应用 |
| Merck Millipore |
Anti-5-methylcytosine, clone 33D3 |
100 µg |
MABE146 |
DB, IF, Southern Blotting, ICC, IH(P), FACS, MeDIP |
| Merck Millipore |
Anti-Methylcytosine dioxygenase TET1 |
100 μg |
09-872 |
WB, ChIP-seq |
| Merck Millipore |
ANTI-DNMT3A2 Antibody (C-Terminus) |
100 µg |
07-2050 |
ICC, ELISA, WB |
| Merck Millipore |
Anti-DNMT1 |
100 µl |
07-688 |
WB |
| Merck Millipore |
ANTI-Phospho-Dnmt1-pS714 |
100 µg |
07-1594 |
WB, ELISA, DB |
| Merck Millipore |
DNMT1,CHKX-100UG |
100 µg |
AB3429 |
WB |
其他相关产品
| 供应商 |
产品名称 |
规格 |
编号 |
| Merck Millipore |
CpGenome Universal Methylated DNA |
10 µg |
S7821 |
| Merck Millipore |
CpGenome Universal Unmethylated DNA set |
10 µg |
S7822 |
| Merck Millipore |
CpGenome Fast DNA Modification Kit |
25rxn |
S7824 |
| Merck Millipore |
CPGENOME DNA MODIFICATION KIT |
100 units |
S7820 |
组蛋白/染色质研究试剂
在表观遗传学的研究内容中， "组蛋白密码"是其重要部分。核心组蛋白上的共价修饰，在真核细胞的染色质结构重塑和基因表达调控方面起重要作用
染色质免疫共沉淀试剂盒
染色质免疫沉淀（ChIP）是研究蛋白质-DNA相互作用的一项强大技术，广泛用于多个领域的染色质相关蛋白的研究（如组蛋白及其异构体，转录因子等），特别适用于已知启动子序列或整个基因位点的组蛋白修饰分析研究。这项技术采用特定抗体来富集存在组蛋白修饰或者转录调控的DNA片段，通过多种下游检测技术（定量PCR，芯片，测序等）来检测此富集片段的DNA序列。
| 供应商 |
产品名称 |
规格 |
编号 |
| Merck Millipore |
CHROMATIN IP (CHIP) ASSAY KIT |
22 Assays |
17-295 |
| Merck Millipore |
EZ CHIP KIT 22 ASSAYS |
22 Assays |
17-371 |
| Merck Millipore |
EZ-Magna ChIP™ A Chromatin Immunoprecipitation Kit |
22 Assays |
17-408 |
| Merck Millipore |
EZ-Magna ChIP™ G Chromatin Immunoprecipitation Kit |
22 Assays |
17-409 |
| Merck Millipore |
Enzymatic ChIP Kit |
1 kit(22 rxns) |
17-375 |
| Merck Millipore |
Acetyl-HistoneH3 ChIPAssay Kit |
22 Assays |
17-245 |
| Merck Millipore |
Acetyl-HistoneH4ChIPAssay Kit |
22 Assays |
17-229 |
| Merck Millipore |
EZ-Magna RIP Kit |
12 rxns |
17-701 |
| Merck Millipore |
Magna RIP Kit |
12 rxns |
17-700 |
| Merck Millipore |
Magna RIP Kit |
48 rxns |
17-704 |
| Merck Millipore |
Protein G Agarose/Salmon Sperm DNA |
2.5 mL |
16-201 |
| Merck Millipore |
Protein A agarose/Salmon Sperm DNA |
2.5 mL |
16-157 |
| Merck Millipore |
Magna ChIP™ Protein A + Protein G Magnetic Beads |
50 reactions |
16-663 |
| Merck Millipore |
Magna ChIP™ Protein A Magnetic Beads |
50 reactions |
16-661 |
| Merck Millipore |
Magna ChIP™ Protein G Magnetic Beads |
50 reactions |
16-662 |
转录因子活性分析试剂盒
转录因子为DNA结合蛋白，严格控制基因的表达。转录因子一般由两个不同的结构域组成，一个结构域与特定DNA序列有高的亲和力，另一个结构域具转录活性。磷酸化特定氨基酸残基或加工结合的抑制性蛋白，可以激活转录因子。研究和定量分析转录因子活性对于研究细胞分化、免疫应答、炎症反应和多种疾病状态等细胞功能至关重要。
Millipore的EZ-TFA转录因子分析试剂盒，结合了EMSA和ELISA的原理，采用显色或化学发光检测，比同位素的方法更快，更容易，更灵敏，更安全，试剂盒检测原理：1. 生物素标记的具有目标转录因子结合保守序列的双链寡核苷酸与细胞/核提取物和TFA实验缓冲液混合孵育；2. 孵育期间，细胞/核提取物中有活性的目标转录因子将结合寡核苷酸保守序列；3. 混合物转移到亲和素包被的96孔上有活性的目标转绿因子通过生物素标记的寡核苷酸被固定在板子上；4. 无活性的未结合的转录因子被洗去；5. 目标转录因子的活性通过抗体、HRP标记二抗和底物反应经化学发光或显色反应被检测。
| 供应商 |
产品名称 |
规格 |
编号 |
| Merck Millipore |
c-Jun EZ-TFA Transcription Factor Assay Colorimetric Kit |
96 assays |
70-540 |
| Merck Millipore |
AP-1 Family EZ-TFA Transcription Factor Assay Colorimetric |
192 assays |
70-550 |
| Merck Millipore |
EZ-TFA Trancription Factor Assay Colorimetric Kit |
96 assays |
70-545 |
| Merck Millipore |
Jun/Fos EZ-TFA Transcription Factor Assay Colorimetric Kit |
96 assays |
70-546 |
| Merck Millipore |
EZ-TFA NFkB p50 Colorimetric |
96 assays |
70-515 |
| Merck Millipore |
NFκB Family EZ-TFA Transcription Factor Assay Colorimetric Kit |
192 assays |
70-560 |
| Merck Millipore |
NFkappaB p50/p65 EZ-TFA Transcription Factor Assay Colorimetric |
96 assays |
70-510 |
| Merck Millipore |
NFκB p65 EZ-TFA Transcription Factor Assay Colorimetric Kit |
96 assays |
70-520 |
| Merck Millipore |
c-Fos EZ-TFA Transcription Factor Assay Chemiluminescent Kit |
96 assays |
70-645 |
| Merck Millipore |
Jun/Fos EZ-TFA Transcription Factor Assay Chemiluminescent Kit |
96 assays |
70-646 |
| Merck Millipore |
c-Jun EZ-TFA Transcription Factor Assay Chemiluminescent Kit |
96 assays |
70-640 |
| Merck Millipore |
NFκB Family EZ-TFA Transcription Factor Assay Chemiluminescent Kit |
192 assays |
70-660 |
| Merck Millipore |
plateNFκB p50 EZ-TFA Transcription Factor Assay (Chemiluminescent) |
96 assays |
70-615 |
| Merck Millipore |
NFkappaB p50/p65 EZ-TFA Transcription Factor Assay (Chemiluminescent) |
96 assays |
70-610 |
| Merck Millipore |
NFκB p65 EZ-TFA Transcription Factor Assay |
96 assays |
70-620 |
蛋白修饰及检测试剂盒
许多蛋白的功能和活化都受到翻译后修饰的调控，一些功能基团的增减可导致蛋白构象的改变，从而使其能够与其他蛋白结合。磷酸化、甲基化、乙酰、泛素化以及SUMO化参与调节蛋白的活性以及信号转导。
| 供应商 |
产品名称 |
规格 |
编号 |
| Merck Millipore |
HDAC Assay Kit, Fluorometric Detection |
96 assays |
17-356 |
| Merck Millipore |
HDAC Assay Kit, Colorimetric Detection |
96 assays |
17-374 |
| Merck Millipore |
HDAC Assay Kit, Fluorometric Detection |
384 assays |
17-372 |
| Merck Millipore |
SIRTainty™ Class III HDAC Assay |
96 assays |
17-10090 |
组蛋白及相关抗体
| 供应商 |
产品名称 |
规格 |
编号 |
应用 |
| Millipore |
ChIPAb+ HDAC1 antibody/primer set |
10 assays |
17-608 |
ChIP, WB |
| Millipore |
ChIPAb+ Acetyl-HistoneH4 antibody/primer set |
25 assays |
17-630 |
ChIP, WB, ICC |
| Millipore |
ChIPAb+ RNA PolII antibody/primer set |
25 assays |
17-620 |
ChIP, WB |
| Millipore |
ChIPAb+ Acetyl-HistoneH3 antibody/primer set |
125 μg |
17-615 |
ChIP, WB, ICC |
| Millipore |
ChIPAb+ Trimethyl-HistoneH3 (Lys9) antibody/primer set |
25 assays |
17-625 |
ChIP, WB |
| Millipore |
ChIPAb+ Trimethyl-HistoneH3 (Lys27) antibody/primer |
25 assays |
17-622 |
ChIP, WB |
| Millipore |
Anti-acetyl Histone H4 (Lys12) |
100 µL |
06-1352 |
WB, ICC |
| Millipore |
Anti-Histone H4, pan 100ul |
100 µL |
05-858 |
ChIP,WB,Mplex, ChIP-seq |
| Millipore |
Anti-trimethyl Histone H3 (Lys27) |
100 μg |
ABE44 |
WB, ICC, PIA,DB |
| Millipore |
Anti-dimethyl Histone H3 (Lys4) |
100 µL |
ABE250 |
WB, ICC, DB, ChIP |
| Millipore |
Anti-acetyl-Histone H2A.X (Lys5) |
100 μg |
AB10020 |
WB, Mplex |
| Millipore |
Anti-acetyl-HistoneH4 |
200 µg |
06-598 |
ChIP, ICC, IP, WB |
| Millipore |
Anti-acetyl-HistoneH4 |
200 µL |
06-866 |
ChIP, WB, ICC |
| Millipore |
Anti-hyperacetylated-HistoneH4 (Penta) |
200 µL |
06-946 |
ChIP, ICC, WB |
| Millipore |
Anti-acetyl-HistoneH4 (Lys5) |
100 µL |
07-327 |
WB, DB, ICC, ChIP-seq, ChIP |
| Millipore |
Anti-acetyl-HistoneH4 (Lys8) |
100 μL |
07-328 |
ChIP, IP, WB, DB, ChIP-seq |
| Millipore |
Anti-acetyl-HistoneH4 (Lys12) |
100 μL |
07-595 |
ChIP, DB, WB, ChIP-seq |
| Millipore |
Anti-acetyl-HistoneH4 (Lys16) |
100 µL |
07-329 |
ChIP, WB, Mplex, PIA, DB, ChIP-seq |
| Millipore |
Anti-monomethyl-HistoneH4 (Lys20) |
200 µg |
07-748 |
WB, Mplex, ICC |
| Millipore |
Anti-dimethyl-HistoneH4 (Lys20) |
100 µL |
07-031 |
ChIP, IF, WB, Mplex, ICC |
| Millipore |
Anti-dimethyl-HistoneH4 (Lys20) |
200 μL |
07-367 |
ChIP, IF, WB, ICC |
| Millipore |
Anti-dimethyl-HistoneH4 (Lys20) |
200 µg |
07-747 |
WB |
| Millipore |
Anti-trimethyl-HistoneH4 (Lys20) |
100 μg |
07-463 |
IP, WB, ChIP, DB |
| Millipore |
Anti-monomethyl-HistoneH3 (Lys4) |
200 µg |
07-436 |
ICC, IF, PIA, WB, ChIP-seq, DB |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys4) |
200 µL |
07-030 |
ChIP, DB, ICC, IF, WB, PIA, ChIP-seq |
| Millipore |
Anti-trimethyl-HistoneH3 (Lys4), clone MC315 |
100 µL |
05-745 |
WB, ChIP, DB, Mplex, ChIP-seq |
| Millipore |
Anti-monomethyl-HistoneH3 (Lys9) |
100 µg |
07-450 |
DB, ICC, PIA, WB |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys9) |
100 µg |
07-441 |
DB, ICC, PIA, WB |
| Millipore |
Anti-trimethyl-HistoneH3 (Lys9) |
100 µg |
07-442 |
DB, ICC, Mplex, PIA, WB, ChIP-seq |
| Millipore |
Anti-monomethyl-HistoneH3 (Lys27) |
200 µg |
07-448 |
DB, ICC, WB, ChIP, ChIP-seq |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys27) |
200 μg |
07-452 |
Mplex, Cell Function Assay, WB, ChIP-seq, DB |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys27) |
100 µL |
07-421 |
ChIP, WB |
| Millipore |
Anti-trimethyl-HistoneH3 (Lys27) |
200 µg |
07-449 |
ICC, IP, Mplex, WB, ChIP-seq |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys36) |
200 µL |
07-274 |
WB, DB |
| Millipore |
Anti-dimethyl-HistoneH3 (Lys36) |
100 µL |
07-369 |
ChIP, WB |
| Millipore |
Anti-dimethyl-Histone H3 (Lys79) |
100 μL |
04-835 |
ChIP, WB, DB, Mplex |
| Millipore |
Anti-HistoneH3 |
100 µg |
06-755
|
ChIP, ICC, WB |
| Millipore |
Anti-acetyl-HistoneH3 |
100 μg |
06-599 |
WB, ICC, ChIP, ChIP-seq |
| Millipore |
Anti-acetyl-HistoneH3 (Lys9) |
100 µL |
07-352
|
ChIP, WB, Mplex |
| Millipore |
Anti-phospho-HistoneH3 (Ser10) |
100 µL |
04-817 |
ChIP, IF, WB, ICC, ChIP-seq, DB, Mplex |
| Millipore |
Anti-phospho(Ser10)-acetyl (Lys14)-Histone H3
|
100 µL |
07-081 |
ChIP, ICC, WB, ChIP-seq |
| Millipore |
Anti-acetyl-HistoneH3 (Lys14) |
100 µL |
07-353
|
ChIP, WB, ChIP-seq, DB, Mplex |
| Millipore |
Anti-acetyl-HistoneH3 (Lys9/18) |
200 μL |
07-593 |
ChIP, WB, Mplex |
| Millipore |
Anti-acetyl-HistoneH3 (Lys18)
|
100 μL |
07-354 |
ChIP, WB |
| Millipore |
Anti-acetyl-HistoneH3 (Lys23) |
100 µL |
07-355
|
ChIP, WB |
| Millipore |
Anti-acetyl-HistoneH3 (Lys27) |
100 μL |
07-360 |
ChIP, WB, Mplex |
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