染色质分析和甲基化测序试剂盒 即CAMe-Seq 试剂盒 货号：A-P-1048
EpiQuik Chromatin Accessibility and Methylome Sequencing (CAMe-Seq) Kit
产品背景：The accessibility of regulatory elements in chromatin is critical for many aspects of gene regulation. Nucleosomes positioned over regulatory elements inhibit access of transcription factors to DNA. To elucidate the role of the interactions between chromatin and transcription factors, it is crucial to determine chromatin accessibility through mapping of the nucleosome positioning along the genome. DNA methylation occurs by the covalent addition of a methyl group at the 5-carbon of the cytosine ring, resulting in 5-methylcytosine. When a CpG island in the promoter region of a gene is methylated, expression of the gene is repressed. It has been demonstrated that nucleosome positioning influences DNA methylation patterning throughout the genome. Thus, obtaining DNA methylation and nucleosome positioning information from the same DNA molecule would enable us to better understand how DNA methylation and nucleosome positioning work together to control chromatin accessibility that regulates gene expression.
Background Information
The EpiQuik™ Chromatin Accessibility and Methylome Sequencing (CAMe-Seq) Kit is a complete set of optimized reagents designed for conducting a gene-specific or genome-wide analysis of both chromatin accessibility and DNA methylation simultaneously from various biological samples at a single molecule or population level, followed by bisulfite-sequencing or next generation sequencing.
The kit has the following advantages and features:
Extremely fast and convenient protocol that allows the entire procedure (from cell tissue sample to ready-to-use DNA for cloning/library preparation) to be finished in as short as 5 hours.
Simultaneously detect chromatin accessibility (nucleosome/transcription factor positioning) and CpG methylation at a base-resolution level.
Internal control primers are included in the kit for validating whether the proper enzymatic DNA methylation and bisulfite modification are achieved.
Recommended procedures for cloning and sequencing are included.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| EpiQuik CAMe-Seq 试剂盒 |
48 次 |
PDF |
A-P-1048-48 |
 |
| EpiQuik CAMe-Seq 试剂盒 |
96 次 |
PDF |
A-P-1048-96 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒--热销中 |
48 次 |
PDF |
A-P-1014B-48 |
|
| 总DNA甲基化定量试剂盒--热销中 |
96 次 |
PDF |
A-P-1014B-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPI研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)，货号是:A-P-1037/A-P-1036。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
