土壤微生物DNA中量提取试剂盒(离心柱型) 货号：A-D6101
ZR Soil Microbe DNA MidiPrep™
背景资料：The ZR Soil Microbe DNA MidiPrep™ is designed for the simple and rapid isolation of humic-free, PCR-quality DNA from microbes in soil. This product can be used to isolate DNA from tough-to-lyse bacteria, fungi, protozoa, and algae that inhabit a variety of samples including clay, sandy, silty, peaty, chalky, and loamy soils. Soil microbes are rapidly and efficiently lysed by bead beating with our state of the art, ultra-high density BashingBeads™. Fast-Spin column technology is then used to isolate the DNA, which is subsequently filtered to remove humic acids/polyphenols that can inhibit PCR. The procedure can be performed in minutes, and there is no need for organic denaturants or proteinases.
产品特点：
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Format
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Spin Column |
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DNA Recovery
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Typically, up to ~125 µg total DNA is eluted into ≥ 150 µl DNA Elution Buffer per sample. |
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Processing Time
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20 min |
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Equipment
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Centrifuge, Vacuum Source and Manifold, Microcentrifuge, Cell Disrupter/Pulverizer w/ 50 ml Tube Adapter |
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DNA Size Limits
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Capable of recovering genomic DNA sized fragments from 100 bp to ≥40 kb. Typical fragment sizes range from 25 to 35 kb. If present, parasitic and viral DNA will also be recovered. |
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Sample Sources
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DNA is isolated from bacteria, fungi, protozoa, and algae in up to 5 g of soil. However, 2.5 g is recommended for most applications. The amount of sample will vary depending on the composition of the sample: process more material for wet muddy samples and less for dry sandy samples. DNA can also be isolated directly from isolated (pelleted) fungus and bacteria (see protocol). |
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DNA Purity
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High quality, humic/fulvic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/280 >1.8, A260/230 >2.0 |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 土壤微生物DNA中量提取试剂盒(离心柱型) |
25 次 |
PDF |
A-D6101 |
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100次 |
PDF |
A-D4002 |
|
| 土壤基因组DNA快速提取试剂盒(离心柱型) |
200次 |
PDF |
A-D4003 |
|
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