基因组DNA提取试剂盒(磁珠法) 货号：A-D3004
ZymoBead™ Genomic DNA Kit
背景资料：The ZymoBead™ Genomic DNA Kit is a simple procedure for the rapid isolation of total DNA (e.g., genomic, mitochondrial, viral) from a variety of biological sample sources. This product has been optimized for maximal recovery of ultra-pure DNA without RNA contamination and is compatible with whole blood (fresh or stored), serum, plasma, buffy coat, solid tissue, bone marrow and buccal cells, cells from culture, and many biological liquid samples. For processing, simply add the specially formulated Genomic Lysis Buffer to a sample in a 1.5 ml tube, add ZymoBeads™, vortex, then centrifuge. There is no need for organic denaturants or Proteinase K digestion because of the unique chemistries featured in the kit that yield high-quality, purified DNA in just minutes (see below). PCR inhibitors are effectively removed during the purification process. DNA purified using the ZymoBead™ Genomic DNA Kit is suitable for PCR, nucleotide blotting, DNA sequencing, endonuclease digestion, bisulfite conversion/methylation analysis, and other downstream applications.
产品特点：
|
Format
|
Spin Column |
|
DNA Recovery
|
Typically, up to 25 µg total DNA is eluted into ≥ 25 µl DNA Elution Buffer per sample. |
|
Processing Time
|
15 min |
|
Equipment
|
Microcentrifuge, vortex, cell disruptor/pulverizer (optional). |
|
DNA Size Limits
|
Capable of recovering genomic DNA up to and above 40 kb. In most instances, mitochondrial DNA and viral DNA (if present) will also be recovered. |
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Sample Sources
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DNA is isolated from bacteria, fungi, protozoa, and algae in up to 0.25 g of soil or from fungal/bacterial cells directly. |
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DNA Purity
|
High-quality, humic-free DNA is eluted with DNA Elution Buffer making it perfect for PCR. A260/A280 >1.8 |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 基因组DNA提取试剂盒(磁珠法) |
400 次 |
PDF |
A-D3005 |
 |
| 基因组DNA提取试剂盒(磁珠法) |
100次 |
PDF |
A-D3004 |
|
| 土壤基因组DNA快速提取试剂盒(离心柱型) |
200次 |
PDF |
A-D4003 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
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| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
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| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
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A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
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A-P-1034-96 |
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| 通用DNA甲基化定量试剂盒 |
48 次 |
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A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
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| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
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