R-Zymolyase - Yeast Lytic Enzyme 货号：A-E1006
背景资料：Digestion of yeast and fungal cell walls is necessary for many experimental procedures including spheroplasting, immunofluorescence, transformation, protein purification, and others. The use of lytic enzymes like Zymolyase is routinely used for digestion. The Zymolyase from Zymo Research is prepared from Arthrobacter luteus, lyophilized, and packaged with a resuspension buffer. The buffer has been optimized to confer maximal levels of enzymatic activity. The main activities of the enzyme are β-1,3 glucanase and β-1,3-glucan laminaripentao-hydrolase, which hydrolyze glucose polymers at the β-1,3-glucan linkages releasing laminaripentaose as the principal product. Optimal Zymolyase activity is at 30° - 37°C; lytic activity ceases at higher temperatures. R-Zymolyase includes 0.5 U/µl RNase A when reconstituted. Susceptible fungal genera: Asbya, Candida, Debaryomyces, Eremothecium, Endomyces, Hansenula, Hanseniaspora, Kloekera, Kluyveromyces, Lipomyces, Metschikowia, Pichia, Pullularia, Saccharomyces, Saccharomycodes, Saccharomycopsis, Schizosaccahromyces, Torulopsis.
产品特点：
|
Format
|
Lyophilized enzyme provided w/ storage buffer |
|
Concentration
|
5 U/µl |
|
Source
|
Arthrobactor luteus |
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Storage
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Zymolyase is stable for over 1 year at -20°C or many years below -70°C. |
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Unit Definition
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One lytic unit is defined as catalyzing a 10% decrease in optical density at 800 nm (OD800) at 30°C in 30 minutes. |
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Optimum pH and Temperature
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pH 7.5, 35°C (lysis of viable yeast), pH 6.5, 45°C (hydrolysis of yeast glucan). |
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Activity
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ß-1,3-glucan laminaripentaohydrolase and ß-1,3-glucanase (trace amount of protease, ca. 1.5 units per 10 µl). DNase and RNase: none detectable. |
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Protein Content
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~10-15 mg/ml |
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Deactivation
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Lytic activity is lost in 5 minutes at 60°C. |
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Assay Condition
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50 mM potassium phosphate, pH 7.5, 10 mM 2-mercaptoethanol in 1 ml yeast cell suspension of 0.8 - 1.0 OD800. |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| R-Zymolyase - Yeast Lytic Enzyme |
1000 Units |
PDF |
A-E1006 |
 |
| Zymolyase - Yeast Lytic Enzyme |
2000 Units |
PDF |
A-E1005 |
|
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200次 |
PDF |
A-D2001-200 |
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