GpC Methylase (M.CviPI) 货号：A-E2014
背景资料：Zymo Research's GpC Methylase completely methylates all cytosines (C5) within a 5'…GpC…3'. context in double-stranded DNA. The enzyme is specific for both non-methylated and hemimethylated DNA. The recombinant GpC Methylase is isolated from an E. Coli strain that expresses the methyltransferase gene from Chlorella virus. The reaction conditions are optimized to maximize the processivity of the enzyme to ensure rapid, complete, and reproducible methylation of DNA for accurate DNA methylation analysis. This product is supplied with 10X Reaction Buffer and S-adenosylmethionine cofactor.
产品描述：For complete, in vitro methylation of DNA for methylation analysis.
Methylation of chromatin DNA for DNA accessibility studies.
Inhibition of endonucleases with overlapping GpC sequence recognition.
[3H]-labeling of DNA.
产品特点：
|
Format
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Provided in solution (4 units/µl) w/ 10X Reaction Buffer and 20X SAM |
|
Source
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Recombinant GpC Methylase is isolated from E. Coli expressing the methyltransferase gene from a Chlorella virus. |
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Storage
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Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage is at ≤ -70°C. |
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Enzyme Commission Number
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(EC 2.1.1.37) |
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Unit Definition
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One unit is defined as the amount of enzyme required to "protect" 1 µg of λ DNA against cleavage by HaeIII restriction endonuclease in a total reaction volume of 20 µl for 1 hour at 37°C. |
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Reaction Conditions
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GpC Methylase in 1X GpC Reaction Buffer w/ 600 µM SAM. Incubate reaction mixtures at 37°C. |
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Inactivation
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Heat-inactivate the enzyme at 65°C for 20 minutes. |
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Standard Reaction Setup
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The reaction volumes can be adjusted accordingly depending on experimental requirements (see protocol). |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| GpC Methylase (M.CviPI) |
200 Units |
PDF |
A-E2014 |
 |
| GpC Methylase (M.CviPI) |
1000 Units |
PDF |
A-E2015 |
|
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