DNA Degradase Plus™ 货号：A-E2020
背景资料：DNA Degradase Plus™ from Zymo Research is a nuclease mix that quickly and efficiently degrades DNA to its individual nucleoside components. Since nucleosides lack negatively charged phosphate, DNA Degradase Plus™ is ideal for whole-genome DNA methylation analysis by LC/MS. Digestion with the enzyme is performed via a one-step procedure that is faster and simpler than other available methods.
产品描述：Quick and simple procedure for generating single nucleosides from DNA for quantitative analysis via LC/MS .
Convenient 1 hour, single-enzyme digest vs. conventional 6-16 hours multi-step enzyme digestion protocols
产品特点：
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Format
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Provided in solution (5 units/µl) w/ 10X Reaction Buffer |
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Storage
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Store at -20°C for up to 12 months. Avoid repeated freeze/thawing of reagents. Prolonged storage should be ≤ -70°C. |
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Unit Definition
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One unit (1 U) is defined as the amount of enzyme required to degrade 1 µg of λ DNA in a total reaction volume of 25 µl for 1 hour at 37°C. |
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Reaction Conditions
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DNA Degradase Plus™ in 1X DNA Degradase™ Reaction Buffer. Incubate reaction mixtures at 37°C for ≥1 hour. |
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Inactivation
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Heat-inactivate at 70°C for 20 minutes.The setup (below) is an example of a typical DNA Degradase Plus™ reaction in a 25 µl final volume. |
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Standard Reaction Setup
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| 2 µl DNA at 500 ng/µl |
| 2.5 µl 10X DNA Degradase™ Reaction Buffer |
| 1 µl DNA Degradase Plus™ (5 units/µl) |
| 19.5 µl ddH2O |
| 25 µl Total Volume |
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| Incubate at 37°C for 2 hours. |
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保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA Degradase Plus™ |
250 Units |
PDF |
A-E2020 |
 |
| DNA Degradase Plus™ |
1000Units |
PDF |
A-E2021 |
|
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