ZR-96 ChIP DNA Clean & Concentrator™ 货号:A-D5206
背景资料:The ZR-96 ChIP DNA Clean & Concentrator™ (ZR-96 ChIP DCC™) provides a hassle-free method for the rapid, high throughput purification and concentration of high-quality DNA from any step in a "standard" ChIP protocol. This includes samples that have: A) undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical/enzymatically-mediated DNA shearing or B) reverse cross-linked samples eluted from chromatin-antibody-bead complexes. Additionally, this product may also be used to purify DNA from PCR and other enzymatic reactions. DNA purified using the ZR-96 ChIP DCC™ is suitable for PCR, Next-Gen sequencing (ChIP-Seq), arrays, as well as other molecular applications.
产品描述:Rapid high throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K digested samples, PCRs, and other enzymatic reactions.
Plate design permits DNA elution at high concentrations into minimal volumes (≥10 µl/well).
Omits the use of organic solvents and the need for ethanol precipitation.
Eluted DNA is well suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.
产品特点:
DNA Recovery
|
Typically, up to 5 µg total DNA (per well) can be eluted into as little as 10 µl of low salt DNA Elution Buffer or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%. |
DNA Size Limits
|
From ~50 bp to 23 kb. |
Sample Sources
|
Any step in a standard ChIP protocol including:
a) Samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical or enzymatically-mediated DNA shearing.
b) Reverse cross-linked samples eluted from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS.
Note: This kit can also be used for DNA purification from PCR, restriction digests, kinase, phosphatase and other enzymatic reactions. |
DNA Purity
|
High-quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications. |
Product Detergent Tolerance
|
≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS. |
保存建议:请在接收到ZR-96 ChIP DNA Clean & Concentrator™产品后,按照产品操作说明书对试剂盒内不同组份进行适当的保存。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
ZR-96 ChIP DNA Clean & Concentrator™ |
2 x 96 次 |
PDF |
A-D5206 |
|
ZR-96 ChIP DNA Clean & Concentrator™ |
4 x 96 次 |
PDF |
A-D5207 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
|
通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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