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  ZR-96 ChIP DNA Clean & Concentrator™ (A-D5206)
   

ZR-96 ChIP DNA Clean & Concentrator™                                                                      货号:A-D5206

背景资料:The ZR-96 ChIP DNA Clean & Concentrator™ (ZR-96 ChIP DCC™) provides a hassle-free method for the rapid, high throughput purification and concentration of high-quality DNA from any step in a "standard" ChIP protocol.  This includes samples that have: A) undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical/enzymatically-mediated DNA shearing or B) reverse cross-linked samples eluted from chromatin-antibody-bead complexes.  Additionally, this product may also be used to purify DNA from PCR and other enzymatic reactions.  DNA purified using the ZR-96 ChIP DCC™ is suitable for PCR, Next-Gen sequencing (ChIP-Seq), arrays, as well as other molecular applications.

 

产品描述:Rapid high throughput (96-well) recovery of ultra-pure DNA from chromatin immunoprecipitation (ChIP), cell lysates, Proteinase K digested samples, PCRs, and other enzymatic reactions. 

Plate design permits DNA elution at high concentrations into minimal volumes (≥10 µl/well).

Omits the use of organic solvents and the need for ethanol precipitation.

Eluted DNA is well suited for use in PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.

产品特点:

DNA Recovery

Typically, up to 5 µg total DNA (per well) can be eluted into as little as 10 µl of low salt DNA Elution Buffer or water. For DNA 50 bp to 10 kb, the recovery is 70-90%. For DNA 11 kb to 23 kb, the recovery is 50-70%.

DNA Size Limits

From ~50 bp to 23 kb.

Sample Sources

Any step in a standard ChIP protocol including:
a) Samples that have undergone reverse cross-linking and Proteinase K or RNase A digestion following mechanical or enzymatically-mediated DNA shearing.
b) Reverse cross-linked samples eluted from chromatin-antibody-bead complexes in TES, 0.1M NaHCO3 and 1% SDS, or other buffers containing up to 1% SDS.
Note: This kit can also be used for DNA purification from PCR, restriction digests, kinase, phosphatase and other enzymatic reactions.

DNA Purity

High-quality, purified DNA is eluted with elution buffer or water and is especially well suited for PCR, Next-Gen sequencing (ChIP-Seq), microarrays, Southern blot analysis, and other molecular applications.

Product Detergent Tolerance

≤5% Triton X-100, ≤5% Tween-20, ≤5% Sarkosyl, ≤1% SDS.

保存建议:请在接收到ZR-96 ChIP DNA Clean & Concentrator™产品后,按照产品操作说明书对试剂盒内不同组份进行适当的保存。

定购信息:

产品名称 规格 操作手册 货号 询价
ZR-96 ChIP DNA Clean & Concentrator™ 2 x 96 次 PDF A-D5206 点击这里给我发消息
ZR-96 ChIP DNA Clean & Concentrator™ 4 x 96 次 PDF A-D5207  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化试剂盒(定性分析) 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化试剂盒(定性分析) 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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