Direct-zol™ RNA MiniPrep w/ TRI-Reagent® 货号:A-R2051
背景资料:The Direct-zol™ RNA MiniPrep provides a streamlined method for the purification of up to 50 µg (per prep) of high-quality RNA directly from samples in TRI-Reagent® or similar*. Total RNA (including small and non-coding RNAs) is effectively isolated from a variety of sample sources (cells, tissues, biological liquids, etc.) using this product. The procedure is easy: simply apply a sample in TRI-Reagent® to spin column and then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. Small RNAs (≥17 nucleotides) are efficiently and consistently recovered using this kit. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
The entire procedure typically takes about 5-10 minutes.
产品描述:Quick, spin column purification of high-quality total RNA directly from TRI-Reagent®, TRIzol®, or similar.
Bypasses phase separation and precipitation procedures.
DNase I included for DNA-free RNA
产品特点:
Format
|
Spin Column |
Processing Time
|
10 min |
Equipment
|
Microcentrifuge |
Sample Sources
|
Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI-Reagent®, TRIzol®, or similar. |
RNA Purity
|
High quality RNA (A260/A280 >1.8, A260/A230 >1.8) is recovered. |
RNA Recovery
|
The RNA binding capacity of the supplied Zymo-Spin™ IIC Column is ~50 µg with a minimal elution volume of 25 µl. |
Compatibility
|
TRI-Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure, RNA-Bee or similar acid-guanidinium-phenol based solutions. |
RNA Storage
|
≤-70 ºC. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage. |
RNA Size Limits
|
RNAs ≥17 nucleotides. |
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
Direct-zol™ RNA MiniPrep w/ TRI-Reagent® |
50 次 |
PDF |
A-R2051 |
|
Direct-zol™ RNA MiniPrep w/ TRI-Reagent® |
200次 |
PDF |
A-R2053 |
|
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200次 |
PDF |
A-D4003 |
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产品名称 |
规格 |
操作手册 |
货号 |
询价 |
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48 次 |
PDF |
A-P-1037-48 |
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96 次 |
PDF |
A-P-1037-96 |
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A-P-1036-48 |
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A-P-1035-48 |
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A-P-1011-48 |
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A-P-1011-96 |
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A-P-1021-48 |
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