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  Direct-zol™-96 RNA (A-R2054)
   

Direct-zol™ RNA MiniPrep                                                                   货号:A-R2054


背景资料:The Direct-zol™-96 RNA provides a streamlined method for the purification of up to 10 µg (per well) of high-quality RNA directly from samples in TRI Reagent®. Total RNA, including small RNAs (17-200 nt), is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product. The extraction method inactivates viruses and other infectious agents. The procedure is easy: simply apply a sample in TRI Reagent® to the Zymo-Spin™ I­96 Plate, then spin, wash, and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.

产品描述:Quick, 96-well purification of high-quality total RNA directly from TRIzol®, TRI Reagent®, or similar.

Bypasses phase separation and precipitation procedures.

DNase I included for DNA-free RNA

产品特点:

Equipment

Centrifuge with microplate carriers.

Sample Sources

Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents.

Inactivation

TRI Reagent® (provided only with R2055 & R2057) inhibits RNase activity and inactivates viruses and other infectious agents.

RNA Purity

A260/A280 > 1.8, A260/A230 > 1.8. Complete removal of DNA can be performed with DNase I digestion.

RNA Recovery

Typically, RNA is eluted into ≥10 µl DNase/RNase-free water allowing for a highly concentrated sample. The RNA binding capacity of the Zymo-Spin™ I-96 Plate is 10 µg/well.

Compatibility

TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol based solutions can be used in place of TRI Reagent®.

RNA Storage

RNA eluted with DNase/RNase-Free Water (provided) can be stored at ≤-70 ºC. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage.

RNA Size Limits

RNAs ≥17 nucleotides.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
Direct-zol™-96 RNA 2x96 次 PDF A-R2054 点击这里给我发消息
Direct-zol™-96 RNA 4x96 次 PDF A-R2056  
Direct-zol™-96 RNA w/ TRI Reagent® 2x96 次 PDF A-R2055  
Direct-zol™-96 RNA w/ TRI Reagent® 4x96 次 PDF A-R2057  
土壤基因组DNA快速提取试剂盒(离心柱型)    200次 PDF A-D4003  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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