Quick-RNA™ MiniPrep 货号：A-R1054
背景资料：The Quick-RNA™ MiniPrep kit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell (up to 107) and tissue samples (up to 50 mg). The procedure combines a unique buffer system with FastSpin column technology to yield high quality total RNA (including small RNAs 17-200 nt) in about 10 minutes.
The procedure is simple: Add the provided RNA Lysis Buffer to a sample, then purify the RNA using the Zymo-Spin™ Columns. The result is highly-concentrated, DNAfree RNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencing etc. In addition, the kit can be used for the enrichment of small and large RNAs into separate fractions.
产品描述：High-quality total RNA from a wide range of samples – single to 107 cells.
Isolate small and large RNAs into separate fractions (optional).
DNA-free RNA for use in any downstream application. DNase I included.
产品特点：
|
Sample Sources
|
Cells or tissue samples, yeast, plant, bacteria, buccal cells, buffy coat, plasma, serum, and other biological liquids. Compatible with RNA Shield™ (Cat. No. R1100) and RNAlater™. |
|
RNA Purity
|
High quality RNA (A260/A280 >1.8, A260/A230 >1.8) suitable for all downstream RNA-based manipulations. |
|
RNA Recovery
|
Up to 100 µg RNA can be eluted into ≥30 µl RNase-free water allowing for a highly concentrated sample. |
|
Sample Size
|
Up to 107 cells or 50 mg tissue. |
|
RNA Storage
|
RNA is eluted with RNase-free water and can be stored frozen. RNase inhibitors can be included for prolonged storage. |
|
Equipment Needed
|
Microcentrifuge |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Quick-RNA™ MiniPrep |
50 次 |
PDF |
A-R1054 |
 |
| Quick-RNA™ MiniPrep |
200 次 |
PDF |
A-R1055 |
|
| Direct-zol™-96 RNA w/ TRI Reagent® |
2x96 次 |
PDF |
A-R2055 |
|
| Direct-zol™-96 RNA w/ TRI Reagent® |
4x96 次 |
PDF |
A-R2057 |
|
| 土壤基因组DNA快速提取试剂盒(离心柱型) |
200次 |
PDF |
A-D4003 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
