Direct-zol-96 MagBead RNA w/ TRI Reagent 货号：A-R2101
背景资料：The Direct-zol™-96 MagBead RNA provides a high-throughput, magnetic bead based purification of high-quality RNA directly from samples in TRI Reagent® and similar. The extraction method inactivates viruses and other infectious agents. Total RNA including small and non-coding RNAs (17-200 nt) is effectively isolated from a variety of sample sources (cells, tissues, serum, plasma, blood, biological liquids, etc.) using this product.
The procedure is easy: simply add Direct-zol™ Binding Buffer and MagBinding Beads to a sample in TRI Reagent®, wash and elute the RNA. No phase separation, precipitation, or post-purification steps are necessary. The result is broad range purification of small and large RNAs suitable for subsequent RNA-based methods including RT-PCR, transcription profiling, hybridization, etc.
产品描述：High-throughput, magnetic bead based purification of high-quality (DNA-free) total RNA directly from TRIzol®, TRI Reagent® and all other acid-guanidinium-phenol based reagents (RNAzol®, QIAzol®, TriPure™, TriSure™, etc.).
Bypasses phase separation and precipitation procedures.
Efficient, broad range purification of small and large RNAs from cells, tissues, biological liquids, in vitro transcripts, etc.
Ideal for viral inactivation/sample storage.
产品特点：
|
Sample Sources
|
Cells from culture, solid tissue, plasma, serum, whole blood, and in vitro processed RNA (e.g., transcription products, DNase-treated or labeled RNA) or samples stored and preserved in TRI Reagent®, TRIzol®, RNAzol®, QIAzol®, TriPure™, TriSure™ and all other acid-guanidinium-phenol reagents. |
|
RNA Purity
|
A260/A280 >1.8, A260/A230 >1.8. Complete removal of DNA is performed with DNase I digestion |
|
RNA Recovery
|
The RNA binding capacity is ~5 µg/20 µl MagBinding Beads. |
|
RNA Storage
|
RNA eluted with the DNase/RNase-Free Water (provided) can be stored at ≤-70°C. The addition of RNase inhibitors (optional) is highly recommended for prolonged storage. |
|
Equipment Needed
|
Automated liquid handler with heating element, plate shaker, and 96-well magnetic stand (not provided). The procedure can also be performed manually. |
|
RNA Size
|
RNAs ≥17 nucleotides. |
|
Sample Inactivation
|
TRI Reagent® (provided only with R2101, R2103, and R2105) inhibits RNase activity and inactivates viruses and other infectious agents. |
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| Direct-zol-96 MagBead RNA w/ TRI Reagent |
2x96 次 |
PDF |
A-R2101 |
 |
| Direct-zol-96 MagBead RNA w/ TRI Reagent |
4x96 次 |
PDF |
A-R2103 |
|
| Direct-zol-96 MagBead RNA w/ TRI Reagent |
8x96 次 |
PDF |
A-R2105 |
|
| Direct-zol™-96 RNA w/ TRI Reagent® |
2x96 次 |
PDF |
A-R2055 |
|
| Direct-zol™-96 RNA w/ TRI Reagent® |
4x96 次 |
PDF |
A-R2057 |
|
| 土壤基因组DNA快速提取试剂盒(离心柱型) |
200次 |
PDF |
A-D4003 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
