组织/细胞自动化DNA提取试剂盒(磁珠法) 货号：A-DM110
Solar® MagSi Tissue DNA Kit
背景资料：Isolation of genomic DNA from tissue or cultured cells with paramagnetic beads.
The Solar.® MagSi Tissue DNA Kit allows rapid and reliable isolation of high-quality genomic DNA from <30 mg tissue or <1 x 107 cultured cells. This kit allows single or multiple simultaneous processing of samples in under 1 hour. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, or precipitation with alcohols. DNA purified using the Solar® Mag-Bind® Tissue DNA Kit is ready for applications such as PCR, Southern blotting, and Restriction enzyme digestion. This kit can be used in conjunction with automated work stations.
产品描述：The Solar® Mag-Si® Tissue DNA Kit provides a rapid and easy method for the isolation of genomic DNA for consistent PCR and Southern analysis. Up to 10 mg tissue or up to 1 cm sections of mouse tail can be readily processed in one time. The method can also be used for preparation of genomic DNA from mouse tail snips, blood, buffy coat, serum, and plasma. The kit allows single or multiple, simultaneous processing of samples. There is no need for phenol/chloroform extractions and time-consuming steps such as precipitation with isopropanol or ethanol are eliminated. The Kit allows single or multiple simultaneous processing of samples in under 1 hour. DNA purified using The Solar® Mag-Si® Tissue DNA Kit is ready for applications such as PCR, Southern blotting and restriction digestion.
产品特点：
1·Safe - No phenol/chloroform extractions；
2·Quality - Complete removal of contaminants and inhibitors；
3·Reliable - Consistent yields at unsurpassed quality ；
4.High Purity - DNA can be used for PCR and real time PCR.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 组织/细胞自动化DNA提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM110-00 |
 |
| 组织/细胞自动化DNA提取试剂盒(磁珠法) |
200次 |
PDF |
A-DM110-01 |
|
| 组织/细胞自动化DNA提取试剂盒(磁珠法) |
500次 |
PDF |
A-DM110-02 |
|
| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
