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  ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法) (A-DMK07)
   

ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法)                                                     货号:A-DMK07

Solar® Mag-Bind® Plant DNA KF Kit II

背景资料:  Isolation of 96 plant DNA with magnetic beads in a 96-well plate format

The Solar® Mag-Bind® Plant DNA KF Kit II is designed for the purification of DNA from plant leaves, roots and seed tissues in a 96-well format. This kit uses paramagnetic particles, considered as mobile solid phase. Unlike column-based systems, the binding of nucleic acids to magnetic particles occurs in solution, resulting in increased binding kinetics and binding efficiency. Particles are completely resuspended during the wash steps of the purification protocol. Thus enhancing contact with the wash buffer, the removal of contaminants, and increases nucleic acid purity. The streamlined protocol can be easily adapted in most robotic liquid handling platform.Purified DNA can be used in PCR as well as more demanding applications such as RAPD.

产品描述:If using the Solar 96® Mag-Bind® Plant KF DNA Kit for the first time, please read this booklet to become familiar with the procedures. Dry or fresh plant tissue is disrupted and lysed in a specially formulated buffer by using liquid nitrogen or automate tissue grinder. Proteins, polysaccharides, and cellular debris are subsequently precipitated. The lysate is transferred to a 96-well microplate, binding conditions are adjusted, and genomic DNA binds to the Mag-Bind® particles. One or two rapid wash steps remove trace contaminants such as residual polysaccharides. And finally pure DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.

产品特点:

Safe - No phenol/chloroform extractions

Automation - Protocol can be easily adapted to robotic workstations

Quality - Complete removal of contaminants and inhibitors

4.Reliable - Consistent yields at unsurpassed quality .

5.High Purity - DNA can be used for most downstream applications.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法) 50 次 PDF A-DMK07-00 点击这里给我发消息
ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法) 2x96 次 PDF A-DMK07-01  
ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法) 8x96 次 PDF A-DMK07-02  
ThermoFisher96孔植物自动化DNA提取试剂盒 II (磁珠法) 20x96次 PDF A-DMK07-03  
组织切片DNA极速提取试剂盒 100 次 PDF A-P-1003-100  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 
 
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