粪便自动化DNA提取试剂盒(磁珠法) 货号：A-DM910
Solar® Mag-Bind® Stool DNA Kit
背景资料： Isolation of genomic DNA from stool samples using paramagnetic Beads
产品描述：If using the Solar® Yeast DNA Kit for the first time, please read this manual before beginning the procedure. Yeast cells are grown to log-phase and spheroblasts are subsequently prepared. Following lysis, binding conditions are adjusted and the sample applied to a HiBind® DNA Mini column. Two rapid wash steps remove trace salt and protein contaminants and finally DNA is eluted in water or low ionic strength buffer. Purified DNA can be directly used in downstream applications without the need for further purification.
产品特点：
1·Fast - Rapid isolation of high quality, ready-to-use genomic DNA；
2·Quality - Highly structurally intact and humic-free DNA；
3·Reliable - Simple and efficient isolation of DNA from soil microbes ；
4.Automation - Protocol can be easily adapted on robotic workstations.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 粪便自动化DNA提取试剂盒(磁珠法) |
5 次 |
PDF |
A-DM910-00 |
 |
| 粪便自动化DNA提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM910-01 |
|
| 粪便自动化DNA提取试剂盒(磁珠法) |
200 次 |
PDF |
A-DM910-02 |
|
| 植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
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| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
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A-P-1036-48 |
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| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
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A-P-1036-96 |
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| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
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| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
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| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
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| DNA甲基化定量试剂盒(比色法) |
96 次 |
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A-P-1034-96 |
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| 通用DNA甲基化定量试剂盒 |
48 次 |
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A-P-1011-48 |
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| 通用DNA甲基化定量试剂盒 |
96 次 |
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A-P-1011-96 |
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| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
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| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
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| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
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| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。
By combining Solar Bio-tek’s novel and proprietary Stool DNA method with the high-performance Mag-Bind® technology, the Mag-Bind® Stool DNA Kit provides a fast and easy method for isolation of genomic DNA from stool samples containing high humic acid contents. The purified DNA is ready to use in most downstream applications such as PCR, Southern blot, SNP analysis. Stool samples are added to a bead beating tube for rapid and thorough homogenization, after lysis, proteins and unsoluble particles was removed. Binding conditions are then adjusted and Mag-Bind® particles are added to bind DNA. After two rapid washing steps to remove trace of contaminants, pure DNA is eluted with Elution Buffer or water. Purified DNA can be directly used in downstream applications without any further purification.
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