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  PCR产物自动纯化试剂盒(磁珠法) (A-DM123)
   

PCR产物自动纯化试剂盒(磁珠法)                                                              货号:A-DM123

Solar® Mag-Bind®Solar Pure Kit

背景资料:Isolate highly concentrated DNA from PCR Products with paramagnetic beads.

产品描述:The Mag-Bind® Solar Pure paramagnetic particles technology provides a better solution for nucleic acid purification compared to centrifugation and vacuum based technologies. The product can be easily scaled up while providing very user-friendly handling procedures. If using the Mag-Bind® Solar Pure for the first time, please read this booklet to become familiar with the procedures. PCR products are first mixed with the Mag-Bind® Solar Pure. PCR products then selectively bind to the Mag-Bind® Solar Pure particles. With one rapid wash step, trace contaminants such as nucleotides, primers and small, nontargeted amplification products are removed. Pure DNA is eluted in Elution Buffer or water. Purified DNA can be directly used in downstream applications without the need for further purification.

产品特点:

Efficiently removes excess primers, primer-dimers, dNTPs and salts

No centrifugation/filtration steps

Scalable - can be adapted to most standard liquid handling robots

4.Use in 96 or 384 well format.

 

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
PCR产物自动纯化试剂盒(磁珠法) 5 ml PDF A-DM123-00 点击这里给我发消息
PCR产物自动纯化试剂盒(磁珠法) 50 ml PDF A-DM123-01  
PCR产物自动纯化试剂盒(磁珠法) 500 ml PDF A-DM123-02  
总RNA自动化富集纯化mRNA试剂盒(磁珠法) 30 次 PDF A-RM911-01  
植物自动化DNA大量提取试剂盒(磁珠法) 50 次 PDF A-DM311-01  
组织切片DNA极速提取试剂盒 100 次 PDF A-P-1003-100  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。

 

The Mag-Bind® Cycle-Pure Kit procedure utilizes a magnetic beads baseds purification method to recover highly concentrated DNA from PCR products. The Mag-Bind® Cycle-Pure Kit is ideally designed for automated purification of PCR samples. By using the highly efficient binding ability of Mag-Bind® technology, DNA fragments are selectively bound to the surface of Mag-Bind® particles.
Salts and other impurities are washed away with two quick wash steps. This kit is suitable for both manual and fully automated processing. Purified DNA is ready for downstream applications include microarrays, automated fluorescent DNA sequencing, and restriction enzyme digestion

 
 
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