凝胶DNA纯化回收试剂盒 货号：A-D2110
Solar® Gel Extraction Kit
背景资料：Recover DNA fragments from agarose gels.
Gel purification of DNA is a common technique used for the isolation of specific DNA fragments from reaction mixtures. However, most methods either fail to completely remove the agarose (which can lead to problems in downstream manipulations), shear the DNA, or results in very low yields. The Solar.® Gel Extraction kit uses HiBind® DNA spin-column technology to recover DNA bands 100bp-20 kb in length from all grades of agarose gel with yields up to 85%. The DNA band of interest is excised from the gel, dissolved in BDP Buffer, and applied to a DNA HiBind® spin column. Following three wash steps, DNA is eluted with deionized water or EB Buffer and is ready for downstream applications
such as ligations, PCR amplification, restriction enzyme digestion and various labeling reactions. This kit can be also used to purify DNA fragments from PCR products and enzymatic reactions. Two spin column formats are are available for this kit.
产品描述：The Solar® family of products is an innovative system that radically simplifies the extraction and purification of nucleic acids from a variety of sources. The key to this system is the new HiBind® matrix that specifically, but reversibly, binds DNA or RNA under certain optimal conditions allowing proteins and other contaminants to be removed. Nucleic acids are easily eluted with deionized water or a low salt buffer.
产品特点：
1·Speed - DNA recovery from agarose gel in 15 min；
2·Reliability - Optimized buffers guarantee pure DNA；
3·Quality - Purified DNA suitable for most applications；
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 凝胶DNA纯化回收试剂盒 |
5 次 |
PDF |
A-D2110-00 |
 |
| 凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
|
| 凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
|
| 凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
|
| 凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
|
| 胶自动纯化回收试剂盒(磁珠法) |
500 次 |
PDF |
A-DM136-02 |
|
| 总RNA自动化富集纯化mRNA试剂盒(磁珠法) |
30 次 |
PDF |
A-RM911-01 |
|
| 植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
| 组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
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关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
