丙烯酰胺凝胶DNA纯化回收试剂盒 货号：A-D2310
DNA purification from acrylamide gels
背景资料：Recover DNA fragments from agarose gels.
The Solar Poly-Gel DNA Extraction Kit provides a fast and efficient method to purify the single or double stranded DNA fragments from polyacrylamides gel. Following the PAGE, the DNA fragments are first visualized by UV shadowing or ethidium bromide staining. The gel slice containing the DNA bands of interest are cut and incubated with a special elution buffer to elute DNA from gel. Polyacrylamide gel is efficiently removed with poly-gel filter column. The sample is then mixed with a special formulated buffer and loaded into the HiBind DNA spin column. After two quick washing steps, purified DNA fragments can be eluted from HiBind DNA spin column with low salt Elution Buffer or water. Purified DNA is ready for downstream applications such as sequencing, ligations, PCR amplification, restriction enzyme digestion.
产品描述：While advances in oligonucleotide synthesis have allowed rapid, facile and efficient production of DNA fragments, purification can be time-consuming and occasionally ineffective. In some applications, there is no need for purified product. However, when purification is necessary, the two traditional techniques used for the purification of synthetic oligonucleotides or double stranded DNA fragments ≤ 100 bp are polyacrylamide gel electrophoresis (PAGE) and high performance liquid chromatography (HPLC). While HPLC remains a potent analytical tool, PAGE and its distant cousin capillary electrophoresis (CE) are probably most often used for the analysis of synthetic oligonucleotides. With UV shadowing (or ethidium bromide staining), PAGE allows the rapid purification of small quantities of synthetic DNA and is especially useful for parallel purification of several samples.
产品特点：
1·Elution Volume: 10-15ul；
2·Generation of highly concentrated DNA；
3·10ug DNA binding capacity；
4.Rapid isolation - less than 15 minutes.
保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分
定购信息：
| 产品名称 |
规格 |
操作手册 |
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