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  总DNA/RNA/Protein共提取试剂盒 (A-RD111)
   

总DNA/RNA/Protein共提取试剂盒                                                                               货号:A-RD111

Solar® Total DNA/RNA/Protein Isolation Kit

背景资料:Simultaneous isolation of genomic DNA RNA and Protein from the same cells or tissues.

Solar® DNA/RNA/Protein Isolation Kit is designed to purify total RNA and genomic DNA simultaneously from a single biological sample source. Up to 100 μg of total RNA and DNA can be isolated from cultured eukaryotic cells and tissues. The kit allows single or multiple, simultaneous processing of samples in less than 30 min. Normally, 1 x 106 - 1 x 107 eukaryotic cells, or 20-30 mg tissue can be used in a single experiment. There is no need for phenol/chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifugation, and precipitation with isopropanol or LiCl, are eliminated. RNA purified using the this method is ready for applications such as RT-PCR*, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation. DNA
purified from this methods can be used for most of downstream applications such as PCR, Southernblot, and genotyping
.

产品描述:The Solar® DNA/RNA/Protein Isolation Kit uses the reversible binding properties of HiBind® matrix, a new silica-based material, combined with the speed of mini-column spin DNA/RNA isolation technology to isolate high quality of DNA and RNA from single sample source. Samples are first lysed and homogenized with a specifically formulated high salt buffer which will immediately inactive the nucleases to ensure the intact of DNA and RNA. The lysate is then passed through the HiBind® DNA column which will bind genomic DNA. After two quick wash steps, the pure, ready-to-use DNA is eluted with low salt ER Buffer or water. The flow-through lysate after the HiBind DNA column is collected and mixed with ethanol to create proper binding condition for RNA. The sample is then loaded to a HiBind RNA column, where the RNA is bound to the membrane and the contaminants are efficiently washed way. Purified RNA is then eluted with is finally eluted in DEPC-treated sterile water.

产品特点:

Convenient - Isolation of both DNA and RNA from the same sample

Fast - Short and streamline protocol delivery Ready-to-use DNA and RNA

Quality - complete removal of contaminants and inhibitors

4.Safe - No organic extractions;

5.Speed - DNA and RNA isolation in less than 30 min.

保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分

定购信息:

产品名称 规格 操作手册 货号 询价
总DNA/RNA/Protein共提取试剂盒 5 次 PDF A-RD111-00 点击这里给我发消息
总DNA/RNA/Protein共提取试剂盒 100 次 PDF A-RD111-01  
总DNA/RNA/Protein共提取试剂盒 200 次 PDF A-RD111-02  
凝胶DNA纯化回收试剂盒 100 次 PDF A-D2110-01  
凝胶DNA纯化回收试剂盒 200 次 PDF A-D2110-02  
凝胶DNA纯化回收试剂盒 500 次 PDF A-D2110-03  
凝胶DNA纯化回收试剂盒 1000次 PDF A-D2110-04  
胶自动纯化回收试剂盒(磁珠法)    150 次 PDF A-DM136-01  
胶自动纯化回收试剂盒(磁珠法)    500 次 PDF A-DM136-02  
总RNA自动化富集纯化mRNA试剂盒(磁珠法) 30 次 PDF A-RM911-01  
植物自动化DNA大量提取试剂盒(磁珠法) 50 次 PDF A-DM311-01  
组织切片DNA极速提取试剂盒 100 次 PDF A-P-1003-100  

表观遗传学产品全面解决方案列下:

产品名称 规格 操作手册 货号 询价
DNA羟甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1037-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1037-96  
DNA羟甲基化定量试剂盒(比色法) 48 次 PDF A-P-1036-48 点击这里给我发消息
DNA羟甲基化定量试剂盒(比色法) 96 次 PDF A-P-1036-96  
DNA甲基化定量试剂盒(荧光法) 48 次 PDF A-P-1035-48 点击这里给我发消息
DNA甲基化定量试剂盒(荧光法) 96 次 PDF A-P-1035-96  
DNA甲基化定量试剂盒(比色法) 48 次 PDF A-P-1034-48 点击这里给我发消息
DNA甲基化定量试剂盒(比色法) 96 次 PDF A-P-1034-96  
通用DNA甲基化定量试剂盒 48 次 PDF A-P-1011-48 点击这里给我发消息
通用DNA甲基化定量试剂盒 96 次 PDF A-P-1011-96  
超敏DNA甲基化定量试剂盒 48 次 PDF A-P-1021-48 点击这里给我发消息
超敏DNA甲基化定量试剂盒 96 次 PDF A-P-1021-96  
总DNA甲基化定量试剂盒 48 次 PDF A-P-1014-48 点击这里给我发消息
总DNA甲基化定量试剂盒 96 次 PDF A-P-1014-96  

推荐阅读:

关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。 

 

 
 
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