血液DNA提取试剂盒(盐析法) 货号:A-DQ134
Solar® SQ Blood DNA Kit
背景资料:Isolate genomic and mitochondrial DNA from whole blood, buffy coat, or cell samples.
The Solar® SQ Blood DNA Kit provides a reliable method for the isolation of high molecular weight genomic and mitochondrial DNA from variable amounts of fresh and frozen whole blood treated with common anticoagulants such as citrate, EDTA and heparin. In addition, buffy coat, bone marrow or cultured cell samples can also be used. DNA from single or multiple samples simultaneously can be isolated in less than 90 minutes. This solution based procedure can be scaled up from a starting sample volume of 0.1 ml to 20 ml. This kit eliminates phenol/chloroform extractions and time-consuming steps such as CsCl gradient ultracentrifugation. DNA purified using the Solar® SQ Blood DNA Kit is free of contaminants and enzyme inhibitors making it suitable for downstream applications such as PCR, Southern blotting and restriction enzyme digestion.
产品描述:The Solar.® SQ Blood DNA Kit uses a highly efficient solution based system to provide a convenient, fast, reliable and non-toxic method to isolate high molecular weight genomic DNA from whole blood or buffy coat. Red blood cells are first lysed with RBC buffer, followed by lysis of the white blood cells and their nuclei in the QTL Buffer. Cellular proteins are removed by precipitation and high molecular weight genomic DNA will remain in solution. High quality genomic DNA is then purified by isopropanol precipitation.
产品特点:
1·Isolation of high molecular weight genomic DNA;
2·No phenol/chloroform extractions ;
3·Versatility in amount of starting material - DNA purification from 0.1-20 ml whole blood;
4.Process single or multiple samples in less than 90 minutes;
5.Large yields of pure DNA.
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
血液DNA提取试剂盒(盐析法) |
10 ml |
PDF |
A-DQ134-00 |
|
血液DNA提取试剂盒(盐析法) |
50 ml |
PDF |
A-DQ134-01 |
|
血液DNA提取试剂盒(盐析法) |
150 ml |
PDF |
A-DQ134-02 |
|
血液DNA提取试剂盒(盐析法) |
300 ml |
PDF |
A-DQ134-03 |
|
植物DNA小量提取试剂盒 |
50 次 |
PDF |
A-DH813-01 |
|
植物DNA小量提取试剂盒 |
200 次 |
PDF |
A-DH813-02 |
|
凝胶DNA纯化回收试剂盒 |
100 次 |
PDF |
A-D2110-01 |
|
凝胶DNA纯化回收试剂盒 |
200 次 |
PDF |
A-D2110-02 |
|
凝胶DNA纯化回收试剂盒 |
500 次 |
PDF |
A-D2110-03 |
|
凝胶DNA纯化回收试剂盒 |
1000次 |
PDF |
A-D2110-04 |
|
胶自动纯化回收试剂盒(磁珠法) |
150 次 |
PDF |
A-DM136-01 |
|
胶自动纯化回收试剂盒(磁珠法) |
500 次 |
PDF |
A-DM136-02 |
|
总RNA自动化富集纯化mRNA试剂盒(磁珠法) |
30 次 |
PDF |
A-RM911-01 |
|
植物自动化DNA大量提取试剂盒(磁珠法) |
50 次 |
PDF |
A-DM311-01 |
|
组织切片DNA极速提取试剂盒 |
100 次 |
PDF |
A-P-1003-100 |
|
表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
通用DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1011-48 |
|
通用DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1011-96 |
|
超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读:
关于5-hmC
5-hmC是近年来在动物组织中发现的,由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能,有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息,也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前,我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序,这些技术都不适合用来检测5-hmC,因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题,EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶,并且区分5-hmC, 5-mC, 和 C,使得研究者能够重新评估他们的DNA甲基化信息,也能够在新样品中寻找DNA羟甲基化。
|