无内毒素质粒DNA大量制备试剂盒                                                                               货号：A-P1222

Solar® Endo-Free Plasmid Maxi Kit

背景资料：Large Scale Isolation of endotoxin-free plasmid DNA from 50-200 mL of overnight culture for high efficiency transfection.

The Endo-Free Plasmid Midi/Maxi Kits provide an efficient method for the large scale isolation of endotoxin free plasmid DNA. Following lysis, endotoxins are removed from the cleared lysate with the addition of ERO Solution. Plasmid DNA is then further purified by adsorbing to the HiBind® silica membrane. An easy wash step is followed by the elution of the bound plasmid DNA in endotoxin free elution buffer. Up to 200 µg of high quality plasmid DNA can be purified from 30-50 mL of overnight culture (P1222), and up to 1mg from 100-200 mL of overnight culture (P1222). Purified DNA samples are ready to be used in downstream applications such as, automated fluorescent DNA sequencing, restriction enzyme digestion, transfection of mammalian cells, cloning, and PCR amplification.

产品描述：The Solar® Fastfilter Endo-free Plasmid Maxi Kit combines the power of HiBind® technology with the time-tested consistency of alkaline-SDS lysis of bacterial cells to deliver high quality DNA. HiBind® Maxi columns facilitate the binding, washing, and elution steps thus enabling multiple samples to be simultaneously processed. This kit also includes a special filter cartridge, which replaces the centrifugation step following alkaline lysis. Following lysis of the cell, endotoxins are removed from the cleared cell lysate with simple extraction-phase-separation steps. Then DNA is bound to the silica membrane and contaminants are removed with a simple wash step. Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, but up to 600-1200 μg of high copy number plasmid or 50-400 μg of low copy number plasmid can be purified from 200 ml overnight culture. Up to 500 ml culture may be processed when working with low-copy number plasmid. The product is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.

产品特点：

1·Endotoxins efficiently reduced (<0.1 EU/µg)；

2·No resin, slurries, or alcohol precipitation；

3·Clear cell lysate without centrifugation ；

4.Fast, easy and convenient protocols；

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
无内毒素质粒DNA大量制备试剂盒	2 次	PDF	A-P1222-00
无内毒素质粒DNA大量制备试剂盒	6 次	PDF	A-P1222-01
无内毒素质粒DNA大量制备试剂盒	25 次	PDF	A-P1222-02
无内毒素质粒DNA大量制备试剂盒	100 次	PDF	A-P1222-03
大体积PCR产物纯化回收试剂盒	10 次	PDF	A-D2118-01
胶自动纯化回收试剂盒(磁珠法)	150 次	PDF	A-DM136-01
胶自动纯化回收试剂盒(磁珠法)	500 次	PDF	A-DM136-02
总RNA自动化富集纯化mRNA试剂盒(磁珠法)	30 次	PDF	A-RM911-01
植物自动化DNA大量提取试剂盒(磁珠法)	50 次	PDF	A-DM311-01
组织切片DNA极速提取试剂盒	100 次	PDF	A-P-1003-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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