高纯质粒DNA中量制备试剂盒 货号:A-P1141
Solar® HP Plasmid Midi Kit
背景资料:Isolate up to 200 µg high-purity plasmid from endonuclease-rich host cells.
Low yield or degradation often occurs in many large-scale plasmid preparations. An Elevated endonuclease level can cause the degradation of DNA during long term storage. The HP Plamid Midi and Maxi kits provide a ideal solution for this situations.
The HP Plasmid Midi Kit allows for the isolation of up to 200 µg of high quality plasmid DNA from 15-50 mL overnight cultures.
产品描述:Yields vary according to plasmid copy number, E.coli strain, and conditions of growth, but 50 ml of overnight culture in LB medium typically produces 100-250μg high-copy number plasmid or 10-100 μg Low-copy number plasmid. Up to 100 ml culture may be processed when working with low-copy number plasmids. The product is suitable for automated fluorescent DNA sequencing, restriction endonuclease digestion, transfection of mammalian cells, and other manipulations.
The Solar® High Performance plasmid purification system is the modified version of Solar ® plasmid isolation system which is designed specially for those applications when high quality plasmid is required such as transfection, autosequencing, etc. It also suitable for isolating plasmid from bacterial hosts (such as end A+ strains) with high level of endonuclease activity. The plasmid from this system has much better stability for long term storage..
产品特点:
1·Safety - No phenol/chloroform extractions ;
2·Versatility - Spin and vacuum formats available ;
3·Quality - endonuclease not detectable;
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分
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