miRNA提取试剂盒I                                    货号：A-RH850

Solar® miRNA Ioslation Kit I

背景资料：For purification of microRNA and total RNA from wide range of samples including animal tissue, cultured cells, plant, bacteria.

The Solar miRNA Isolation Kit uses a rapid procedure to isolate small RNAs, such as microRNA (miRNA), small interfering RNA (siRNA), and small nuclear RNA (snRNA), from wide range of tissues and cells. The fast and efficient silica membrane based method isolates total RNA ranging in size from kilobases down to 10mers. The kit also provides reagents and a procedure to enrich the population of RNAs that are 200 bases and smaller, which enhances the sensitivity of small RNA detection by solution hybridization, Northern analysis, and other methods.

产品描述：Solar® miRNA Kit combines the s reversible binding properties of HiBind® matrix, a new silica-based, time saving spin technology material, with the efficiency of the time proved phenol/Guanidine-based monophasic solution to purify total RNA from variety of samples. The sample is lysed and homogenized first under highly denaturing buffer contains phenol and Guanidine Thiocyanate (PurZole Reagent) to protect RNA from degradation. After addition of Chloroform, the homogenate is separated into aqueous and organic phases by centrifugation. The upper aqueous phase which contains RNA is extracted and mixed with ethanol to adjust binding condition for large RNA. The sample is loaded into a HiBind® RNA column to which the large RNA molecules including mRNA are bound. The flow-through sample is mixed with ethanol again to create proper binding condition for smaller RNA and loaded into a MicroElute® RNA column to bind smaller RNA molecules. After two brief wash steps, the enriched miRNA and other smaller RNA molecules are eluted with RNase-free water.

产品特点：

1·Efficient isolation of small RNA-containing total RNA ；

2·Simple 30 minute procedure ；

3·Compatible with virtually all cell and tissue types；

4.Enrich for small RNA <200 nt to increase sensitivity in downstream analyses!

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

定购信息：

产品名称	规格	操作手册	货号	询价
miRNA提取试剂盒I	5 次	PDF	A-RH850-00
miRNA提取试剂盒I	50 次	PDF	A-RH850-01
miRNA提取试剂盒I	200 次	PDF	A-RH850-02
通用植物 micro RNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5331-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5901-50
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5901-100
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5311-50
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5311-100
microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5301-50
microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5301-100
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014B-96

RT-PCR相关产品全面解决方案列下:

产品名称	规格	操作手册	货号	询价
Thermo M-MLV 反转录酶	10000U	PDF	A-P6801
Thermo M-MLV 反转录酶	50000U	PDF	A-P6802
Supermo III M-MLV 反转录酶	10000U	PDF	A-P6811
Supermo III M-MLV 反转录酶	50000U	PDF	A-P6812
Super M-MLV 反转录酶	10000U	PDF	A-P6502
Super M-MLV 反转录酶	50000U	PDF	A-P6503

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