通用型microRNA提取试剂盒                                                 货号：A-21300

microRNA Purification Kit

背景资料：Norgen’s microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules from cultured animal cells, small tissue samples, bacterial cells, plants and blood  in as little as 25 minutes. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. miRNAs and siRNAs are typically 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Norgen’s microRNA Purification Kit purifies RNA molecules exclusively smaller than 200 nt, without the use of phenol or chloroform. microRNA could be isolated from a broad range of sample sources including cultured cells, small tissue samples, bacteria, yeast, fungi, plant and bodily fluids.

产品描述：Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. The process involves the use of two different spin columns: the Large RNA Removal Column and the microRNA Enrichment Column.  Briefly, the cells or tissues of interest are lysed using the provided Lysis Buffer and ethanol is added to the sample.  The lysate is then applied to the Large RNA Removal Column, and the larger RNA molecules will bind to Norgen's resin while the smaller RNA species will pass through into the flowthrough (BIND 1).  Ethanol is then added to the flowthrough, the sample is then applied to the microRNA Enrichment Column and the small RNA molecules will bind to Norgen's resin (BIND 2).  Only the RNA will bind to the column, and any remaining impurities are removed through a series of washes (WASH).  The purified small RNA molecules are then eluted into 20 - 50 µL of the provided Elution Buffer or water (ELUTE).  Please see procedure flowchart to the right.

产品特点：

1·Fast and easy processing - Rapid spin-column format allows for the processing of 10 samples in 30 minutes；

2·Streamlined protocol - Efficient isolation of small RNA species using a 2 column process, resulting in minimal contamination of larger RNA and genomic DNA ；

3.No phenol:chloroform extractions - RNA is isolated without the use of harmful chemicals such as phenol or chloroform；

4.Specific small RNA purification - All small RNA species (<200 nt) can be isolated including miRNA, siRNA, tRNA and 5S rRNA；

5.Isolate total RNA from a broad input source - Total RNA has been isolated from cultured animal cells, small tissue samples, bacteria, yeast, fungi, plant and bodily fluid including blood；

6.Optional purification of large RNA molecules - Large RNA molecules >200 nt can also be easily purified using an optional protocol

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

定购信息：

产品名称	规格	操作手册	货号	询价
通用型microRNA提取试剂盒	25 次	PDF	A-21300-1
通用型microRNA提取试剂盒	50 次	PDF	A-21300-2
通用植物 micro RNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5331-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5901-50
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5901-100
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5311-50
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5311-100
microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5301-50
microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5301-100
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014B-96

RT-PCR相关产品全面解决方案列下:

产品名称	规格	操作手册	货号	询价
Thermo M-MLV 反转录酶	10000U	PDF	A-P6801
Thermo M-MLV 反转录酶	50000U	PDF	A-P6802
Supermo III M-MLV 反转录酶	10000U	PDF	A-P6811
Supermo III M-MLV 反转录酶	50000U	PDF	A-P6812
Super M-MLV 反转录酶	10000U	PDF	A-P6502
Super M-MLV 反转录酶	50000U	PDF	A-P6503

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