尿液microRNA提取试剂盒                                                 货号：A-29000

Urine microRNA Purification Kit

背景资料：Norgen’s Urine microRNA Purification Kit provides a rapid method for the isolation and purification of small RNA molecules (< 200 nt) from urine samples. These small RNAs include regulatory RNA molecules such as microRNA (miRNA) and short interfering RNA (siRNA), as well as tRNA and 5S rRNA. Small RNA molecules are often studied due to their ability to regulate gene expression. Typically miRNAs are 20-25 nucleotides long, and regulate gene expression by binding to mRNA molecules and affecting their stability or translation. Several recent studies have shown that miRNA regulates cell growth and apoptosis. Furthermore, clinical and experimental analyses suggested that miRNAs may function as a novel class of oncogenes or tumor suppressor genes. MicroRNA expression profiles of different tumor types, relative to their normal tissues, have recently been shown to provide phenotypic signatures for particular cancer types. Unique patterns of aberrant miRNA expression may serve as molecular biomarkers for tumor diagnosis, prognosis of disease specific outcomes, and prediction of therapeutic responses.

产品描述：Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the urine sample is first lysed using the provided Lysis Solution, ethanol is added and the microRNA is bound to Norgen's column (BIND). Under these conditions only the RNA will bind to Norgen's resin while most of the contaminating cellular proteins are removed in the flowthrough or retained on top of the resin. The bound RNA is then washed to remove any remaining impurities (WASH). Lastly, the purified urinary microRNA is eluted into 50 µL of the provided Elution Buffer or water (ELUTE). Please see procedure flowchart to the right.

产品特点：

1·Purification of all small RNA molecules without the use of phenol；

2·Rapid and convenient spin-column protocol；

3·Isolate inhibitor-free urinary microRNA!

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

定购信息：

产品名称	规格	操作手册	货号	询价
尿液microRNA提取试剂盒	25 次	PDF	A-29000-1
尿液microRNA提取试剂盒	50 次	PDF	A-29000-2
通用植物 micro RNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5331-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5901-50
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5901-100
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5311-50
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5311-100
microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5301-50
microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5301-100
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014B-96

RT-PCR相关产品全面解决方案列下:

产品名称	规格	操作手册	货号	询价
Thermo M-MLV 反转录酶	10000U	PDF	A-P6801
Thermo M-MLV 反转录酶	50000U	PDF	A-P6802
Supermo III M-MLV 反转录酶	10000U	PDF	A-P6811
Supermo III M-MLV 反转录酶	50000U	PDF	A-P6812
Super M-MLV 反转录酶	10000U	PDF	A-P6502
Super M-MLV 反转录酶	50000U	PDF	A-P6503

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