DNA/RNA/microRNA/Protein共提取试剂盒                                                 货号：A-29000

All-in-One Purification Kit

背景资料：Norgen’s All-in-One Purification Kit provides a rapid method for the isolation and purification of total RNA including microRNA, genomic DNA and proteins sequentially from a single sample of cultured animal cells, small tissue samples, blood, bacteria, yeast, fungi or plants. In addition, a microRNA Enrichment Column is provided for the optional separate purification of small RNA (<200 nt), allowing for 4-in-1 purification (large RNA, genomic DNA, microRNA and proteins). This kit is an ideal all-in-one solution for researchers studying systems biology, including those who are interested in the interactions of multiple disciplines including RNA interference, genomics, epigenomics, transcriptomics and proteomics. Norgen’s All-in-One Purification Kit is especially useful for researchers who are isolating macromolecules from precious, difficult to obtain or small samples such as needle biopsies and single foci from cell cultures, as well as for mutant analysis, RNA interference studies and pathogen detection. Furthermore, analysis will be more reliable since the RNA, DNA and proteins are derived from the same sample without any fractionation, thereby eliminating inconsistent results. The purification procedure is very rapid, allowing for the isolation of large RNA, genomic DNA, microRNA and proteins from a single sample in less than 40 minutes. The isolated macromolecules are of the highest purity and can be used in a number of different downstream applications.

产品描述：Purification is based on spin column chromatography using Norgen’s proprietary resin as the separation matrix. Briefly, the process involves first lysing the cells or tissue of interest with the provided Lysis Solution. Ethanol is then added to the lysate, and the solution is loaded onto a spin-column. Under these conditions only the RNA and DNA will bind to Norgen’s resin, while the proteins are removed in the flowthrough (BIND 1).   An additional protocol utilizing the microRNA Enrichment Column is provided if the user wishes to further separate the large (>200 nt) and the small/microRNA (<200 nt). Next, the bound RNA is washed with the provided RNA Wash Solution to remove impurities, and the purified RNA is eluted with the RNA Elution Solution (WASH ELUTE 1A). The remaining bound genomic DNA is then washed with the provided gDNA Wash Buffer to remove any remaining trace levels of RNA, and the gDNA is then eluted with the gDNA Elution Buffer (WASH ELUTE 1B). The kit purifies all sizes of RNA, from large mRNA and ribosomal RNA down to microRNA (miRNA) and small interfering RNA (siRNA). 

产品特点：

1·Purify RNA, microRNA, proteins and DNA from a single sample；

2·No phenol extraction step ；

3·Fast and easy processing；

4.Ideal for precious, difficult to obtain or small samples；

5.Flexible protocol allows for total RNA purification (including microRNA) or separate enrichment of microRNAs!保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分。

定购信息：

产品名称	规格	操作手册	货号	询价
尿液microRNA提取试剂盒	25 次	PDF	A-29000-1
尿液microRNA提取试剂盒	50 次	PDF	A-29000-2
通用植物 micro RNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5331-100

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5901-50
血液（液体样本）microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5901-100
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5311-50
石蜡包埋组织microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5311-100
microRNA极速提取试剂盒(离心柱型)	50 次	PDF	A-R5301-50
microRNA极速提取试剂盒(离心柱型)	100次	PDF	A-R5301-100
通用DNA甲基化定量试剂盒	48 次	PDF	A-P-1011-48
通用DNA甲基化定量试剂盒	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014B-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014B-96

RT-PCR相关产品全面解决方案列下:

产品名称	规格	操作手册	货号	询价
Thermo M-MLV 反转录酶	10000U	PDF	A-P6801
Thermo M-MLV 反转录酶	50000U	PDF	A-P6802
Supermo III M-MLV 反转录酶	10000U	PDF	A-P6811
Supermo III M-MLV 反转录酶	50000U	PDF	A-P6812
Super M-MLV 反转录酶	10000U	PDF	A-P6502
Super M-MLV 反转录酶	50000U	PDF	A-P6503

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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。