植物总RNA提取试剂盒 货号:A-R3201
Solar® Plant RNA Kit
背景资料:Isolation of Total RNA from plant samples.
Solar® Plant RNA Kit provides a convenient and rapid method for the isolation of total RNA from a variety of plant samples. This kit provides a homogenizer column for homogenization and filtration of viscous plant cell lysate by centrifugation in combination with HiBind® RNA spin column for RNA purification. All the contaminants including polysaccharides and phenolic compounds are effectively removed. Purified RNA can be used for most downstream applications such as RT-PCR, Northern blot analysis, differential display and poly A+ RNA selection.
产品描述:This protocol is suitable for most fresh or frozen tissue samples allowing efficient recovery of RNA. However, due to the tremendous variation in water and polysaccharide content of plants, sample size should be limited to ≤100 mg. Best results are obtained with young leaves or needles. The method isolates sufficient RNA for a few tracks on a standard Northern assay. Wearing latex disposable gloves collect tissue in a 1.5-ml or 2-ml microfuge tube and freeze by dipping in liquid nitrogen with a pair of tweezers to fill the tube. Grind the tissue using disposable pestles or equivalent. Alternatively, one can allow liquid nitrogen to evaporate and then store samples at -70oC for later use. Do not allow samples to thaw. Use disposable pestles only once. Alternatively, a small clean mortar and pestle can be used. The above methods for disrupting plant tissue cannot be replaced with mechanical homogenizers.
产品特点:
1· Fast - High quality RNA in 30 minutes。
2·Safe - No organic extractions。
3· Quality - Purified RNA suitable for most applications。
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。
定购信息:
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