通用型总RNA大量提取试剂盒II 货号:A-RH117
Solar® Total RNA Maxi Kit II
背景资料:Isolation of total cellular RNA from all types of animal and plant samples.
The Solar.® Total RNA Maxi Kit II is designed for isolating total cellular RNA from tissues rich fibrous and fatty tissues such as skeletal muscle, heart, brain and adipose tissues. Compared to other standard silica-column procedures, the Solar.® Total RNA Maxi Kit II provides higher yield and better quality of RNA from all types of tissue. This kit combines phenol/guanidine-base lysis and the silica membrane purification of RNA technology to provide a rapid and easy method of the isolation of total RNA from any tissue sample. RNA purified using the Solar.® Total RNA method is ready for applications such as RT-PCR, Northern blotting, poly A+ RNA (mRNA) purification, nuclease protection, and in vitro translation.
产品描述:The Solar® Total RNA Maxi Kit II uses the reversible binding properties of HiBind ® matrix, a new silica-based material. By combined the high lysis efficient of PurZol ® Reagent with Solar innovative HiBind ® technology, this kit can extract total cellular RNA from different sources of samples especially for fatty tissues such as brain and adipose tissue. A specifically formulated high salt buffer system allows more than 5 mg of RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first homogenized with PurZol® Reagent that practically inactivates RNase. After add chloroform, the homogenate is separated into aqueous and organic phase with centrifugation. The aqueous phase which contains RNA then adjusted with ethanol and then applied to the HiBind® spin columns to which total RNA binds, while cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted in DEPC-treated sterile water.
产品特点:
1· High-throughput RNA purification。
2·Fast and convenient sample processing。
3·No organic extraction or precipitation。
4.High-quality RNA for any application。
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。
定购信息:
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规格 |
操作手册 |
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询价 |
通用型总RNA大量提取试剂盒II |
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20 次 |
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表观遗传学产品全面解决方案列下:
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规格 |
操作手册 |
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询价 |
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A-P-1037-48 |
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