微量组织/细胞总RNA提取试剂盒 货号:A-RH854
Solar® MicroElute Total RNA Kit
背景资料:Isolation of RNA from small amounts of samples with lower elution volumes.
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Solar® MicroElute Total RNA Kit provides a rapid and easy method for the isolation of up to 50 µg of total RNA from small amounts of cultured eukaryotic cells, tissues such as as laser dissected samples (LDS) or fine needle aspirates(FNA). Normally, up to 5 x105 eukaryotic cells or 5 mg tissue (amounts depend on the tissue used) can be used in a single experiment. This kit allows processing single or multiple of samples in less than 30 minutes. There is no need for phenol/ chloroform extractions, and time-consuming steps such as CsCl gradient ultracentrifuation, and precipitation with isopropanol or LiCl. Purified RNA can be eluted with 10-15 µl nuclease-free water. Purified RNA is ready for most downstream applications such as RT-PCR, Northern Blotting, Poly A+ purification, Nuclease protection and in vitro translation
产品描述:The Solar® MicroElute® Total RNA Kits combine the reversible binding properties of HiBind® matrix, a new silica-based material with the speed of mini-column spin technology. A specifically formulated high salt buffer system allows RNA molecules greater than 200 bases to bind to the matrix. Cells or tissues are first lysed under denaturing conditions that practically inactivate RNase. After add the ethanol, samples are then applied to the HiBind® MicroElute ® columns to which total RNA binds, while cellular debris and other contaminants are effectively washed away. High quality RNA is finally eluted in DEPC-treated sterile water.
产品特点:
1· Reliable RNA isolation from small samples-microdissected tissues and cells。
2·High quality total RNA concentrated in small volume (10-15 µl)。
3·Maximal yields of RNA from small amount of samples。
保存建议:在接收到艾德寄出的试剂盒后请按照说明书建议,使用不同的保存条件或温度来保存试剂盒内组分。
定购信息:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
微量组织/细胞总RNA提取试剂盒 |
5 次 |
PDF |
A-RH854-00 |
|
微量组织/细胞总RNA提取试剂盒 |
50 次 |
PDF |
A-RH854-01 |
|
微量组织/细胞总RNA提取试剂盒 |
200 次 |
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A-RH854-02 |
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A-RH716-01 |
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表观遗传学产品全面解决方案列下:
产品名称 |
规格 |
操作手册 |
货号 |
询价 |
DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
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96 次 |
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A-P-1037-96 |
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A-P-1036-48 |
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A-P-1011-96 |
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48 次 |
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A-P-1021-48 |
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96 次 |
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A-P-1021-96 |
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48 次 |
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A-P-1014-48 |
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