DNase I (RNase-free)is a nonspecific endonuclease that degrades double- and single-stranded DNA and chromatin. It functions by hydrolyzing phosphodiester linkages, producing mono and oligonucleotides with a 5'-phosphate and a 3'-hydroxyl group. Supplied in one tube of 2,000 U (2 U⁄μL).
RNase-free DNase I is of the highest purity available and is recommended to degrade DNA in the presence of RNA when the absence of RNase is critical to maintain the integrity of the RNA. For example, DNase I is frequently used to remove template DNA following in vitro transcription, and to remove contaminating DNA in total RNA preparations (especially those from transfected cells that may contain plasmid DNA), used for ribonuclease protection assays, cDNA library contraction, and RT-PCR. DNase I requires bivalent cations (Mg2+ and Ca2+ at approximately 5 mM and 0.5 mM, respectively) for maximal activity and has a pH optimum of 7.8.
产品描述：The Solar® SQ Tissue DNA Kit uses a highly efficient solution based system to provide a convenient, fast, reliable and non-toxic method to isolate high molecular weight genomic DNA from various samples. Samples are first lysed with WBL buffer. Cellular proteins are removed by precipitation and high molecular weight genomic DNA will remain in solution. High quality genomic DNA the is purified by isopropanol precipitation.
