DNA文库制备试剂盒(与illumina仪器配合使用)                                                                        货号：A-P-1051

EpiNext DNA Library Preparation Kit (Illumina)

背景资料：DNA library preparation is a critical step for next generation sequencing (NGS). To generate accurate sequencing data for NGS, the prepared library DNA should be sufficient in yield and of high quality. Also, as NGS technology is continuously improving, DNA library preparation is required to be optimized accordingly.  Most of the currently used methods are unfortunately time-consuming, expensive, and inconvenient. Some of the methods are relatively quick by combining end repair and dA tailing or even ligation in one-step, but have been shown to generate significant G tailing or form concatmers at the ligation step or have high insertion bias. These side reactions eventually result in the prepared DNA library being less efficient and inaccurate. An ideal DNA library preparation method should be balanced in speed, convenience, small sample-suitability, cost-effectiveness, and accuracy. To address this issue, Epigentek offers the EpiNext DNA Library Preparation Kit.The EpiNext™ DNA Library Preparation Kit  (Illumina) is a complete set of optimized reagents to carry out a successful DNA library preparation. The kit is suitable for preparing a DNA library for next generation sequencing applications using an Illumina sequencer, which includes genomic DNA-seq, ChIP-seq, MeDIP/hMeDIP-seq, bisulfite-seq, and targeted re-sequencing. The optimized protocol and components of the kit allow the DNA library to be constructed quickly with reduced bias. The kit has the following advantages:

产品描述：This kit incluldes all reagents required at each step to carry out a successful DNA library preparation. In the library preparation, DNA is first fragmented to the appropriate size (about 300 bp peak size). The end repair of the DNA fragments is performed and an A-overhang is added at the 3'-end of each strand.  Adaptors are then ligated to both ends of the end repaired/dA tailed DNA fragments for amplification and sequencing. Fragments are then size selected and purified with MQ beads, which allows for quick and precise size selection of DNA. Size-selected DNA fragments are then amplified with a high-fidelity PCR Mix which ensures maximum yields from minimum amounts of starting material and provides highly accurate amplification of library DNA with low error rates and minimum bias。  

产品特点：

1·Fast and streamlined procedure - the procedure from fragmented DNA to size selection is less than 2 h 30 min.  Only one clean-up between each step, thereby saving time and preventing handling errors, as well as loss of valuable samples. Gel-free size selection further reduces the preparation time；

2·Highly Convenient - the kit contains all required components for each step of DNA library preparation, which are sufficient for end repair, dA tailing, ligation, clean-up, size selection and library amplification, thereby allowing the library preparation to be streamlined with the most reliable and consistent results；

3· Minimized bias - Ultra HiFi amplification and an optional PCR-free step enable the user to achieve reproducibly high yields of DNA library with minimal sequence bias and low error rates ；

4·Flexibility - Uses various dsDNA including fragmented dsDNA isolated from various tissue or cell samples, dsDNA enriched from ChIP reactions, MeDIP/hMeDIP reactions, or exon capture. Broad range of input DNA from 10 ng to 1 µg. PCR-free library preparation can be performed with use of 500 ng or more input DNA；

保存建议：在接收到艾德寄出的试剂盒后请按照说明书建议，使用不同的保存条件或温度来保存试剂盒内组分

定购信息：

产品名称	规格	操作手册	货号	询价
DNA文库制备试剂盒(illumina)	12 次	PDF	A-P-1051-12
DNA文库制备试剂盒(illumina)	24 次	PDF	A-P-1051-24
高敏感亚硫酸盐测序试剂盒(illumina)	12 次	PDF	A-P-1056-12
高敏感亚硫酸盐测序试剂盒(illumina)	24 次	PDF	A-P-1056-24
SETDB1特异基因敲除定量试剂盒-表观遗传调控因子	96 次	PDF	A-P-5002-SETDB1-96
通用乙酰化组蛋白H3K36定量分析试剂盒(荧光法)	96 次	PDF	A-P-4019-96

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

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