Hycult Dx MBL(人) ELISA试剂盒                                                       货号：E-DH001

Hycult Dx Human MBL ELISA kit

背景资料：The Hycult Dx human MBL ELISA is a ready-to-use ligand-lectin solid-phase enzyme-linked immuno assay with a working time of 4 hours· The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples, standards and controls are incubated in microtiter wells coated with mannan· During this incubation the mannan binding property of functional human MBL is used to capture human MBL by solid bound mannan· Biotinylated tracer antibody to human MBL is added to the wells· If functional, mannan binding human MBL is present in the sample, the tracer antibodies will bind to the mannan bound human MBL· Streptavidin-peroxidase will bind to the biotinylated tracer antibody· Streptavidin-peroxidase will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human MBL standards (log)· The human MBL concentration of samples and controls, which are run concurrently with the standards, can be determined from the standard curve。

产品描述：Mannose Binding Lectin (MBL) is a C-type lectin and an important element in innate immunity· MBL belongs to the collectin family of proteins that consists of a collagen-like domain and a carbohydrate recognition domain (CRD)· Through this, MBL recognizes carbohydrates (e·g· mannose and N-acetylglucosamine) on pathogens· MBL forms several sizes of oligomers that are composed of subunits of three identical 32 kDa polypeptides· MBL is a pattern recognition receptor, and upon recognition of the infectious agent, MBL triggers the activation of the lectin-complement pathway through attached C1r/C1s-like serine proteases, designated MASP· The MBL-MASP complex proteolytically cleaves C4, C2 and C3· The lectin pathway is antibody and C1q-independent and is, therefore, independent of the classical and alternative complement activation pathways· MBL is synthesized by hepatocytes and has been isolated from the liver or serum of several vertebrate species· Normal human plasma contains MBL concentrations ranging from 10- to 5,000 ng/ml· Up to 12% of healthy Caucasian blood donors have MBL concentrations below 100 ng/ml· Low plasma concentrations have been associated with an inherited defect in opsonization· The MBL concentration is enhanced in infectious diseases· Measuring of MBL is indicated in recurrent infections (especially in children), primary/secondary immunodeficiencies, artherosclerosis/coronary heart disease, cystic fibrosis, transplantation, autoimmune diseases (SLE/Rheumatoid arthritis) and habitual abortion· MBL measurement is not affected by the presence of antibodies against mannan· In the human MBL assay, any influence of the classical pathway has been eliminated by using a special MBL-binding buffer, which inhibits the binding of C1q to immunocomplexes and disrupts the C1 complex, while leaving the function of the MBL complex intact。 

产品特点：The minimum concentration which can be measured is 0·41 ng/ml。

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
Hycult Dx MBL(人)ELISA试剂盒	1 x 96 det	PDF	E-DH001
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
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DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
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DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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