EndoClear迷你LAL实验包(红色): (EndoTrap red 1/1 + mini LAL)                       货号：E-HIT306

EndoClear, red, small (EndoTrap red 1/1 + mini LAL)

背景资料：Profos EndoTrap red assay (endotoxin removal): Binding capacity of 2,000,000 EU/ml· Endotoxin removal rates of >95% per cycle· Final LPS concentration 0·005 - 0·1 EU/ml· Column or batch mode· Operating at pH 6·0-9·0· Suitable for proteins, peptides and antibodies· Suitable for an ionic strength up to 250 mM NaCl (< 100 mM is recommended)· Suitable for LPS from Klebsiella pneumoniae and Serratia marcescens· Hycult Biotech Mini-LAL assay (endotoxin detection): Minimum concentration which can be measured is 0·01 EU/ml· Measurable endotoxin detection range of 0·01-10 EU/ml· Working volume of 50 ul/well· 72 determinations Operating at pH 6·5-8·0

产品描述：Bacterial endotoxin, lipopolysaccharide (LPS), is a fever-producing by-product of gram-negative bacteria commonly known as pyrogen· LPS consists of a polysaccharide, a core oligosaccharide and lipid A, which is responsible for the toxic effects· In human, lipopolysaccharide binding protein (LBP) plays a central role in response to LPS in the activation pathway and in the neutralization of LPS· Removal of endotoxin is one of the most difficult downstream processes during protein purification· EndoTrap red, as part of the EndoClear kit, is developed to meet the most challenging requirements of endotoxin removal: the highest endotoxin removal capacity combined with excellent recovery rates· EndoTrap red is based on affinity chromatography for column or batch mode· The working range of EndoTrap red is pH 6-9 and it works with an ionic strength up to 250 mM NaCl (<100 mM is recommended)· Proteins, peptides and antibodies can be applied onto the EndoTrap red column· LPS from Klebsiella pneumoniae and Serratia marcescens can only be removed with EndoTrap red· Besides the EndoTrap red, EndoTrap blue is also available· EndoTrap blue has a pH range of 4-9 and is capable to work with an ionic strength up to 600 mM· Endotoxin in plasmid can only be removed with EndoTrap blue· The detection of endotoxin is based on studies of Frederick Bang· He observed that bacteria caused intravascular coagulation in the American horseshoe crab, Limulus polyphemus· In collaboration, Levin and Bang found that the agent responsible for the clotting phenomena resided in the crab’s amoebocytes, or circulating blood cells, and that pyrogen triggers the turbidity and gel-forming reaction enzymatically· The Mini-LAL assay for endotoxin detection, the second part of the EndoClear kit, makes use of an activated enzyme· In the presence of a colorless substrate, the enzymatic reaction will cause a yellow colour to develop upon cleavage of the chromophore, p-nitroaniline (pNA)· The reaction is stopped by the addition of acetic acid and the absorbance at 405 nm is measured with a spectrophotometer· The endotoxin concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined from a standard curve 

产品特点：The EndoClear kit provides the opportunity to remove and detect endotoxin with just one kit· EndoClear consists of the Profos EndoTrap red assay (endotoxin removal) and the Hycult Biotech Mini-LAL assay (endotoxin detection)· The Profos EndoTrap red assay is intended for the removal of endotoxin with removal rates of >95% per cycle· The binding capacity of EndoTrap red is 2,000,000 EU/ml (1EU = 100 pg LPS)

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
EndoClear迷你LAL实验包(红色): (EndoTrap red 1/1 + mini LAL)	1 x  1 ml	PDF	E-HIT306
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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