EndoClear迷你LAL实验包(蓝色)：(EndoTrap 10 + mini LAL)                         货号：E-HIT309

EndoClear, blue, bulk (EndoTrap 10 + mini LAL)

背景资料Profos EndoTrap blue assay (endotoxin removal): The Profos EndoTrap blue Assay is based on affinity chromatography with a working time of ~30 minutes· Column is prepared with the 10 ml settled resin· Sample is applied to the column with equilibration buffer· Fractions are collected· Endotoxin removal rate is >95% per cycle· Samples are measured with Hycult Biotech LAL Assay· Hycult Biotech Mini-LAL assay (endotoxin detection): The LAL assay is an enzyme-based assay with a working time of ~45 minutes· Samples and standards are incubated with LAL reagent· The enzymatic reaction, triggered by endotoxin, will cause a yellow color to develop upon cleavage of the chromophore, p-nitroaniline· The enzymatic reaction is stopped by the addition of acetic acid· The absorbance at 405 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the E· coli standards (log)· The endotoxin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·：

产品描述：Bacterial endotoxin, lipopolysaccharide (LPS), is a fever-producing by-product of gram-negative bacteria commonly known as pyrogen· LPS consists of a polysaccharide, a core oligosaccharide and lipid A, which is responsible for the toxic effects· In human, lipopolysaccharide binding protein (LBP) plays a central role in response to LPS in the activation pathway and in the neutralization of LPS· Removal of endotoxin is one of the most difficult downstream processes during protein purification· EndoTrap blue, as part of the EndoClear kit, is developed to meet the most challenging requirements of endotoxin removal: the highest endotoxin removal capacity combined with excellent recovery rates· EndoTrap blue is based on affinity chromatography for column or batch mode· The working range of EndoTrap blue is pH 4-9 and it works with an ionic strength up to 600 mM NaCl· Proteins, peptides, antibodies and plasmid DNA can be applied onto the EndoTrap blue column· Customer specific buffers can be used when enriched freshly with Ca2+· Besides the EndoTrap blue, EndoTrap red is also available· EndoTrap red has a pH range of 6-9 and is capable to work with an ionic strength up to 250 mM (<100 mM is recommended)· LPS from Klebsiella pneumoniae and Serratia marcescens can only be removed with EndoTrap red· The detection of endotoxin is based on studies of Frederick Bang· He observed that bacteria caused intravascular coagulation in the American horseshoe crab, Limulus polyphemus· In collaboration, Levin and Bang found that the agent responsible for the clotting phenomena resided in the crab’s amoebocytes, or circulating blood cells, and that pyrogen triggers the turbidity and gel-forming reaction enzymatically· The Mini-LAL assay for endotoxin detection, the second part of the EndoClear kit, makes use of an activated enzyme· In the presence of a colorless substrate, the enzymatic reaction will cause a yellow colour to develop upon cleavage of the chromophore, p-nitroaniline (pNA)· The reaction is stopped by the addition of acetic acid and the absorbance at 405 nm is measured with a spectrophotometer· The endotoxin concentration of samples with unknown concentrations, which are run concurrently with the standards, can be determined from a standard curve 

产品特点：The kit has a minimum endotoxin detection limit of 0·01 EU/ml·

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
EndoClear迷你LAL实验包(蓝色)：(EndoTrap 10 + mini LAL)	10 ml	PDF	E-HIT309
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

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