CRP(人)ELISA试剂盒 货号：E-HK358
CRP, Human, ELISA kit
背景资料：The human CRP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 1 hour and 10 minutes· The efficient format of 1 plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human CRP· Peroxidase conjugate antibody will bind to the captured human CRP· Peroxidase conjugate antibody will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of Sulfuric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human CRP standards (log)· The human CRP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·
产品描述：
C-Reactive Protein (CRP) is an acute-phase protein, produced exclusively in the liver· Interleukin-6 is the mediator for the synthesis by the hepatocytes- of CRP, a pentamer of approximately 120·000 Daltons· CRP is present in the serum of normal persons at concentrations ranging up to 5mg/l· The protein is produced by the fetus and the neonate and it does not pas the placental barrier, as such it can be used for the early detection of neonatal sepsis· Because febrile phenoena, leukocyte count and erythrocyte sedimentation rate (ESR) are often misleading, investigators now prefer a quantitative CRP determination as a marker for acute inflammation and tissue necrosis· Within 6 hours of an acute inflammatory challenge the CRP level starts to rise· Serum concentration of CRP increases significantly in cases of both infectious and non-infectious inflammation, of tissue damage and necrosis and in the presence of malignant tumours·-- CRP is present in the active stages of inflammatory disorders like rheumatoid arthritis, ankylosing spondylitis, Reiter’s syndrome, psoriatric arthropathy, systemic lupus erythematosus, polyarteritis, ulcerative colitis and Crohn’s disease·
产品特点：The patient samples are diluted 1:1000 in two consecutive steps: pipette 10 ul of each patient sample into separate glass dilution tubes and add 990 ul of diluted Sample Dilution Buffer· Mix thoroughly· Add 450 ul of diluted Sample Dilution Buffer to 50 ul of these 100 x prediluted samples· Mix thoroughly·
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| CRP(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK358 |
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