C1q(小鼠)ELISA试剂盒 货号：E-HK211-01
C1q, Mouse, ELISA kit
背景资料：The mouse C1q ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing mouse C1q· Biotinylated tracer antibody will bind to the captured mouse C1q· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the mouse C1q standards (log)· The mouse C1q concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·
产品描述： C1q forms together with C1r and C1s the C1 macromolecule, the first component of the classical complement pathway· The formation of an antibodyantigen complex (immune complex) is the principal way of activating the classical pathway of the complement system· C1q triggers the activation process when it docks onto antibodies within these immune complexes· In this way, C1q acts to bridge the innate and adaptive immune systems· Interaction of immune complexes with C1q induces a conformational change within the C1 complex, which results in activation of the classical pathway· C1q functions as recognition unit by binding to the heavy chain of IgG or IgM (Fc gamma and Fc micro) provided that the immunoglobulins are bound to their antigen· Furthermore, C1q can bind to apoptotic blebs, where it activates the classical complement pathway and mediates phagocytosis· As such, C1q promotes the clearance of apoptotic cells and subsequent exposure of auto-antigens, thereby preventing stimulation of the immune system· C1q is predominantly produced by macrophages but also by follicular dendritic cells, interdigitating cells and cells of the monocyte-macrophage lineage· C1q deficiency has a profound effect on host defense and clearance of immune complexes·
产品特点：Detection level 15·6 ng/ml
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| C1q(小鼠)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK211-01 |
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