LL-37(人)ELISA试剂盒 货号：E-HK321
LL-37, Human, ELISA kit
背景资料：The human LL-37 ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human LL-37· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human LL-37 standards (log)· The human LL-37 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··
产品描述：Cathelicidins are a family of antimicrobial proteins predominantly found in the peroxidase-negative granules of neutrophils· The cathelicidins are synthesized as preproproteins· Within the neutrophils, they are stored in granules as inactive proforms after removal of the signal peptide· The active biologic domains of the cathelicidins generally reside in the C-terminus· The C-terminal antimicrobial peptides are activated when cleaved from the proforms of the cathelicidins by serine proteases from azurophil granules· Human cationic antimicrobial protein (hCAP)18 is the only human cathelicidin identified to date· The antibacterial C-terminus of hCAP-18, LL37 (37 amino acids), has been shown to exert broad antimicrobial activity towards gram-negative as well as gram-positive bacteria and to have synergistic antibacterial effects with the defensins· For instance, deficiency in saliva LL37 accords with occurrence of periodontal disease in patients with morbus Kostmann· Moreover, it functions as a chemotactic agent for neutrophils, monocytes and T cells· LL-37 is markedly resistant to proteolytic degradation and to a limited extent also cytotoxic towards mammalian cells· LL-37 was demonstrated to be present in plasma in levels from 25 - 250 ng/ml and to be enhanced in infectious diseases··
产品特点：Detection level 0·14 ng/ml
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| LL-37(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK321-01 |
 |
| LL-37(人)ELISA试剂盒 |
2 x 96 det |
PDF |
E-HK321-02 |
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