精氨酸酶I(人)ELISA试剂盒 货号：E-HK322
Arginase I, Human, ELISA kit
背景资料：The human arginase-I ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human arginase-I· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the arginase-I standards (log)· The human arginase-I concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·
产品描述：Arginase-I (liver-type arginase, L-arginine ureahydrolase, or L-arginine amidinohydrolase) is an hydrolytic enzyme that converts arginine to ornithine and urea· Ornithine is the precursor for preline, an essential amino acid for collagen synthesis and for poly-amines, key components in cell growth and differentiation· Arginase exists as two forms designated arginase-I and arginase-II which are encoded by different genes· Although arginase-I and arginase-II have similar enzyme activities, they differ in pI and immunological reactivity· Human arginase-I (liver-type arginase) is a 35 kD protein circulating in blood probably as a homotrimer· Liver-type arginase is most abundant expressed in mammalian liver, but is also found in non-hepatic tissues, for instance red blood cells, lactating mammalian glands, and the kidney· In addition to its involvement in ammonia detoxification via the urea cycle, arginase plays a role in other processes, for instance macrophage-mediated cytotoxicity due to arginase release and inhibition of lymphocyte proliferation in vitro· It shows high activity in growing tissues, wound healing, proliferating lymphocytes and tumors· Furthermore, arginase acts as a modulator of the immune response· Besides this arginase plays a role in allergen challenged lungs, in autoimmune inflammation in the central nervous system and in acute liver injury· In plasma of healthy individuals arginase-I is present in levels of 1·8 - 30 ng/ml, which increases approximately 10 fold during acute phase responses·
产品特点：Detection level 0·4 ng/ml
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| 精氨酸酶I(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK322-01 |
 |
| 精氨酸酶I(人)ELISA试剂盒 |
2 x 96 det |
PDF |
E-HK322-02 |
|
| VisionBlue快速细胞活力分析试剂盒 |
500 次 |
PDF |
C-K303-500 |
|
表观遗传学产品全面解决方案列下：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| DNA羟甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1037-48 |
|
| DNA羟甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1037-96 |
|
| DNA羟甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1036-48 |
|
| DNA羟甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1036-96 |
|
| DNA甲基化定量试剂盒(荧光法) |
48 次 |
PDF |
A-P-1035-48 |
|
| DNA甲基化定量试剂盒(荧光法) |
96 次 |
PDF |
A-P-1035-96 |
|
| DNA甲基化定量试剂盒(比色法) |
48 次 |
PDF |
A-P-1034-48 |
|
| DNA甲基化定量试剂盒(比色法) |
96 次 |
PDF |
A-P-1034-96 |
|
| 通用DNA甲基化试剂盒(定性分析) |
48 次 |
PDF |
A-P-1011-48 |
|
| 通用DNA甲基化试剂盒(定性分析) |
96 次 |
PDF |
A-P-1011-96 |
|
| 超敏DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1021-48 |
|
| 超敏DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1021-96 |
|
| 总DNA甲基化定量试剂盒 |
48 次 |
PDF |
A-P-1014-48 |
|
| 总DNA甲基化定量试剂盒 |
96 次 |
PDF |
A-P-1014-96 |
|
推荐阅读：
关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC
5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。 |
