MBL/MASP-2(人)ELISA试剂盒                                             货号：E-HK327

MBL/MASP-2, Human, ELISA kit

背景资料：The Hycult Biotech human functional MBL/MASP-2 assay is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle which a working time of 4·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allow free choice of batch size for the assay· After activation samples and standards are captured by solid bound mannan· Human C4 is added to the wells· The C4 binds to the mannan bound functional MBL/MASP-2 complex and is cleaved· HRP-labeled tracer antibody binds to a C4 degradation product bound to the functional MBL· HRP-labeled tracer antibody reacts with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the MBL standards (log)· The functional MBL/MASP-2 concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··

产品描述：The Hycult Biotech human functional MBL/MASP-2 assay has been developed for the quantitative measurement of active human MBL in serum, plasma and culture medium, through the measurement of the ability of MBL/MASP complexes to initiate C4 cleavage when it is bound to mannan· The assay is based on the assumption of the presence of an excess of MASP-2· Since MASP-2 deficiency is very rare this assumption is justifiable· The mannan-binding lectin (MBL) pathway of complement activation has been established as the third pathway of complement activation· MBL is a carbohydrate-binding serum protein, which circulates in complex with serine proteases known as mannan-binding lectin associated serine proteases (MASPs)· When bound to micro-organisms, the MBL complex activates the complement components C4 and C2, thereby generating the C3 convertase and leading to opsonisation by the deposition of C4b and C3b fragments· MBL is able to activate the complement activation pathway independent of the classical and alternative pathways and plays an important role in innate immunity· In this assay any influence of the classical pathway has been eliminated by using a special MBL-binding buffer, which inhibits the binding of C1q to immune complexes and disrupt the C1 complex, while leaving the carbohydrate binding activity of MBL and the integrity of the MBL complex intact· Normal human plasma contains a MBL concentration ranging from 3·3 to 1650 U/ml· Up to 12 % of healthy Caucasian blood donors have MBL levels below 33 U/ml· The MBL level correlates with the amount of C4b-depositing capacity· However, the C4b deposition capacity can vary threefold between individuals with similar MBL concentrations· The Hycult Biotech human functional MBL/MASP-2 assay is useful in evaluating the importance of the MBL pathway in relation to clinical manifestations· Several investigations have reported that low levels of MBL correlate with susceptibility to various infections and diseases·

产品特点：Detection level 0·7 U/ml

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
MBL/MASP-2(人)ELISA试剂盒	1 x 96 det	PDF	E-HK327-01
MBL/MASP-2(人)ELISA试剂盒	2 x 96 det	PDF	E-HK327-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

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