TCC(人)ELISA试剂盒 货号：E-HK328
TCC, Human, ELISA kit
背景资料：The human TCC ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured TCC· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the TCC standards (log)· The TCC concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··
产品描述：The three distinct activation pathways of complement converge with the formation of a C5 convertase and it is cleavage of C5 by these convertases that initiates the lytic or terminal pathway· In contrast to the activation pathways, which require enzymatic cleavage for activation, the terminal pathway relies on conformational changes induced by binding· Binding of C6 facilitates binding of C7 which alters the conformation of the complex· After binding of C8 a variable number of C9 molecules associate with the C5b678 complex, the so called terminal complement complex (TCC) or Membrane Attack Complex (MAC)· The formation of the membrane form of TCC causes lysis of cells or can trigger a variety of cellular metabolic pathways resulting in the synthesis and release of inflammatory mediators· The TCC contains neoantigens that are absent from the individual native components· Neoantigens are present both in the membrane-bound (MAC) and the fluid-phase (SC5b-9) complex· TCC is present in normal human plasma, < 1 AU/ml, and increases in patients with complement activation·
产品特点：Detection level 8·2 mAU/ml
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
询价 |
| TCC(人)ELISA试剂盒 |
1 x 96 det |
PDF |
E-HK328-01 |
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| TCC(人)ELISA试剂盒 |
2 x 96 det |
PDF |
E-HK328-02 |
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C-K303-500 |
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