Lactoferrin(人)ELISA试剂盒                                            货号：E-HK329

Lactoferrin, Human, ELISA kit

背景资料：The human lactoferrin ELISA kit is a solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· Samples and standards are captured by a solid bound specific antibody· Biotinylated tracer antibody will bind to captured human lactoferrin· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of citric acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human lactoferrin standards (log)· The human lactoferrin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·

产品描述：Human lactoferrin (LF) is an 80 kDa glycoprotein found concentrated in the secondary granules of the neutrophils· In addition, lactoferrin can be found in epithelia and most body fluids and secretions· Lactoferrin was first isolated from human milk and plays an important part in the immune system by helping to fight infections· It has the ability to bind iron and possesses five different enzyme activities: DNase, RNase, ATPase, phosphatase, and malto-oligosaccharide hydrolysis· Lactoferrin is a natural anti-bacterial, anti-fungal and anti-viral protein, it is an antioxidant and also possesses immunomodulatory properties· Furthermore, lactoferrin promotes the health of the gastro-intestinal system by improving the intestinal microbalance· Lactoferrin is secreted in plasma by neutrophils· Plasma of healthy individuals contains ~190-500 ng/ml LF· The lactoferrin plasma concentration represents a positive relation to the total pool of neutrophils and the rate of neutrophil turnover· Upon inflammation, lactoferrin is released from the secondary neutrophil granules into the extracellular medium· Therefore, the extracellular lactoferrin concentration can be used as an index for neutrophil activation· The iron binding property of lactoferrin is considered to be an important antimicrobial function· Human lactoferrin binds via its highly positively charged amino-terminus to bacterial products· It kills various bacteria, most probably by inducing intracellular changes in these bacteria without affecting the membrane permeability· Cleavage by pepsin of lactoferrin leads to the release of lactoferricin H· This 47 amino acid peptide has more antimicrobial activity than its precursor and it can inhibit the classical but not the alternative complement pathway· Urine or breast milk of healthy persons contain ~30 ng/ml and ~500 ug/ml LF, respectively· During infection, the LF concentration can raise 10-100-fold· In faeces of healthy persons, ~1 ug/g LF can be detected, whereas in faeces derived from colon cancer or inflammatory bowel disease (IBD) patients, LF levels range from ~75-310 ug/g· Faecal lactoferrin is useful as a sensitive and specific marker in identifying intestinal inflammation such as Crohn’s disease and IBD· Combination of several markers, such as calprotectin, defensin, elastase, MPO, I-FABP and MAdCAM, may be useful for classifying IBD, as well as for identifying tumor grade and to confirm remission/response to treatment· Therefore, the Hycult Biotech Human Lactoferrin ELISA is a sensitive, non-invasive tool for monitoring disease activity·

产品特点：Detection level 0·4 ng/ml

保存建议：推荐4℃保存，有效期一年。

定购信息： 

产品名称	规格	操作手册	货号	询价
Lactoferrin(人)ELISA试剂盒	1 x 96 det	PDF	E-HK329-01
Lactoferrin(人)ELISA试剂盒	2 x 96 det	PDF	E-HK329-02
VisionBlue快速细胞活力分析试剂盒	500 次	PDF	C-K303-500

表观遗传学产品全面解决方案列下：

产品名称	规格	操作手册	货号	询价
DNA羟甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1037-48
DNA羟甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1037-96
DNA羟甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1036-48
DNA羟甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1036-96
DNA甲基化定量试剂盒(荧光法)	48 次	PDF	A-P-1035-48
DNA甲基化定量试剂盒(荧光法)	96 次	PDF	A-P-1035-96
DNA甲基化定量试剂盒(比色法)	48 次	PDF	A-P-1034-48
DNA甲基化定量试剂盒(比色法)	96 次	PDF	A-P-1034-96
通用DNA甲基化试剂盒(定性分析)	48 次	PDF	A-P-1011-48
通用DNA甲基化试剂盒(定性分析)	96 次	PDF	A-P-1011-96
超敏DNA甲基化定量试剂盒	48 次	PDF	A-P-1021-48
超敏DNA甲基化定量试剂盒	96 次	PDF	A-P-1021-96
总DNA甲基化定量试剂盒	48 次	PDF	A-P-1014-48
总DNA甲基化定量试剂盒	96 次	PDF	A-P-1014-96

推荐阅读：

关于5-hmC
5-hmC是近年来在动物组织中发现的，由胞嘧啶修饰而来。5-hmC在表观遗传学上的功能可能与5-甲基化胞嘧啶(5-mC)不同。尽管到现在为止还不确知其功能，有研究者猜测它在调控基因的表达与关闭过程中起着重要的作用。5-mC的发现让我们不得不重新评估DNA甲基信息，也不得不监测人类的健康组织和病理组织之间5-mC相对分布的差异。在EPI公司的MethylFlash技术之前，我们还没有发现任何直接的常规方法来检测5- hmC,以及区分5-hmC和5-mC

5-hmC 和 5-mC的区别
时下常用的DNA甲基化分析方法包括限制内切酶酶切和亚硫酸氢盐或MeDIP介导的MS-PCR和测序，这些技术都不适合用来检测5-hmC，因为它与5-mC事实上很难用这类方法区分开来。为了解决这个问题，EPIk研制了MethylFlash羟甲基化DNA定量试剂盒(荧光法)。本试剂盒提供了一种很经济的方法来检测5-羟甲基化胞嘧啶，并且区分5-hmC, 5-mC, 和 C，使得研究者能够重新评估他们的DNA甲基化信息，也能够在新样品中寻找DNA羟甲基化。