SAP(人)ELISA试剂盒 货号:E-HK331
SAP, Human, ELISA kit
背景资料:The human SAP ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human SAP· Biotinylated tracer antibody will bind to the captured human SAP· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human SAP standards (log)· The human SAP concentration of samples, which are run concurrently with the standards, can be determined from the standard curve··
产品描述:Serum amyloid P component (SAP) belongs to a highly conserved superfamily of calcium-dependent ligand binding and lectin (carbohydrate binding) proteins· Due to the pentameric structure these proteins are also called pentraxins· SAP is 25kDa in size· C-reactive protein (CRP) and SAP are well-charactarized short pentraxins, which are produced in the liver in response to inflammatory mediators· Pentraxin 3 (PTX3) is the prototypic long pentraxin· Both SAP and CRP are evolutionary conserved in all vertebrates and found in distant invertebrates such as the horseshoe crab (Limulus polyphemus)· They share 51% residue sequence identity and 66% homology·
产品特点:Detection level 0·5 ng/ml
保存建议:推荐4℃保存,有效期一年。
定购信息:
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操作手册 |
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