SAA(人)ELISA试剂盒 货号:E-HK333
SAA, Human, ELISA kit
背景资料:The human SAA ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 2·5 hours· The efficient format of 2 plates with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human SAA· Peroxidase conjugated antibody will bind to the captured human SAA· Peroxidase conjugated antibody will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human SAA standards (log)· The human SAA concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·
产品描述:Serum Amyloid A (SAA) is the circulating precursor of amyloid A protein· In humans, four SAA genes have been described· Two genes (SAA1 and SAA2) encode acute phase SAA (A-SAA)· SAA3 is a pseudogene from which no mRNA or protein product has been identified· SAA4 encodes constitutive SAA (C-SAA)· SAA is 20 kDa in size, 12·5 kDa under denaturing conditions· The liver is the primary site of synthesis of SAA· Extra-hepatic production has been demonstrated in macrophages, endothelial cells, epithelial cells, artherosclerotic lesions, tumors and synovial tissue· SAA-1 is the major isoform of SAA in plasma· SAA-1 levels in serum/plasma of healthy individuals range from 1-5 um/ml··
产品特点:Detection level 3·1 ng/ml
保存建议:推荐4℃保存,有效期一年。
定购信息:
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规格 |
操作手册 |
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SAA(人)ELISA试剂盒 |
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