乳铁蛋白(人)ELISA试剂盒 货号：E-HK334
Properdin, Human, ELISA kit
背景资料：The human Properdin ELISA is a ready-to-use solid-phase enzyme-linked immunosorbent assay based on the sandwich principle with a working time of 3·5 hours· The efficient format of a plate with twelve disposable 8-well strips allows free choice of batch size for the assay· Samples and standards are incubated in microtiter wells coated with antibodies recognizing human Properdin· Biotinylated tracer antibody will bind to the captured human Properdin· Streptavidin-peroxidase conjugate will bind to the biotinylated tracer antibody· Streptavidin-peroxidase conjugate will react with the substrate, tetramethylbenzidine (TMB)· The enzyme reaction is stopped by the addition of oxalic acid· The absorbance at 450 nm is measured with a spectrophotometer· A standard curve is obtained by plotting the absorbance (linear) versus the corresponding concentrations of the human Properdin standards (log)· The human Properdin concentration of samples, which are run concurrently with the standards, can be determined from the standard curve·
产品描述：Properdin, or factor P, is a single-chain plasma glycoprotein (apparent molecular mass 52-55 kDa), consisting of six thrombospondin repeat sequences between short N- and C-terminal domains· In the blood it exists as a mixture of head-to-tail dimers, trimers and tetramers, with a preponderance of trimers· The protein is produced by a variety of leukocytes, including monocytes, T lymphocytes and neutrophils , but also by endothelial cells in which properdin synthesis and release are induced by shear stress· Properdin participates in the alternative pathway of complement activation together with C3 and factors B, D, I and H· It stabilizes the labile C3bBb which is deposited on immune complexes or foreign surfaces· This permits amplification of C3bBb formation in competition with catabolism of C3b by factor I, which uses factor H as a cofactor· The local amplification process leads to the creation of the alternative pathway C5 convertase, C3bBb3b, and initiates the terminal pathway of complement activation
产品特点：Detection level 0·31 ng/ml
保存建议：推荐4℃保存，有效期一年。
定购信息：
| 产品名称 |
规格 |
操作手册 |
货号 |
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1 x 96 det |
PDF |
E-HK334-01 |
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| 乳铁蛋白(人)ELISA试剂盒 |
2 x 96 det |
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E-HK334-02 |
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